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PXD024679

PXD024679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn ERK5-KLF2 signalling module regulates early embryonic gene expression dynamics and stem cell rejuvenation
DescriptionCCE mESCs were transfected with either empty pCAGGS vector or HA-MEK5DD (S311D T315D) construct for 48 hours. 24 hours prior to lysis, mESCs were either treated with 10uM AX15836 or DMSO control, and transfected mESCs selected with puromycin. Conditions were performed in triplicate.
HostingRepositoryPRIDE
AnnounceDate2021-03-18
AnnouncementXMLSubmission_2021-03-18_07:55:46.593.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGreg Findlay
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-11 06:21:31ID requested
12021-03-18 07:55:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ERK5-KLF2, signalling, embryonic gene expression, stem cell rejuvenation, TMT, LC-MS, mass spectrometry
Contact List
Greg Findlay
contact affiliationThe MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, the University of Dundee, Dundee DD1 5EH, UK
contact emailg.m.findlay@dundee.ac.uk
lab head
Greg Findlay
contact affiliationMRC-PPU, University of Dundee
contact emailg.m.findlay@dundee.ac.uk
dataset submitter
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Dataset FTP location
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