PXD024670

PXD024670 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals SnRK1-dependent protein in rice sheaths during grain filling
Description	Insufficient leaf photosynthesis promotes sheath NSC transport to the panicle during grain filling. SnRK1 regulates assimilate distribution between plant tissues and organs. However, the mechanism by which SnRK1 regulates sheath NSC transport to the panicle and its response to limited leaf assimilation remain unclear. Here, we performed leaf cutting (LC) at anthesis to simulate insufficient leaf assimilation and set no treatment as a CK. In response to LC, SnRK1 activity increased rapidly, and NSC transport was advanced. Sheath NSCs were not transported in a snrk1a mutant with deficient SnRK1 activity. These results indicated that SnRK1 activity is important for sheath NSC transport. The high correlation of T6P and sucrose and the inhibition of SnRK1 by T6P in sheaths in vitro implied that T6P slightly inhibits SnRK1 activity in rice sheaths in response to sucrose availability. The increase in SnRK1 activity was accompanied by an increase in OsSnRK1a expression and a low sucrose content. Low sucrose signaling increases SnRK1 transcription. Therefore, we speculated that OsSnRK1a expression positively regulates SnRK1 activity in response to low sucrose availability in rice sheaths. Through phosphoproteomics and further PRM verification, 20 phosphosites that depend on SnRK1 and are correlated with NSC transport were obtained. Based on the function of these sites and proteins, we found that SnRK1 regulates starch degradation, sucrose metabolism, phloem transport, sugar transport across tonoplast, and glycolysis via phosphorylation to promote sheath NSC transport. Overall, our results revealed the importance, function and regulatory mechanism of SnRK1 in sheath NSC transport.
HostingRepository	PRIDE
AnnounceDate	2022-03-18
AnnouncementXML	Submission_2022-03-17_18:26:25.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hu Yuxiang
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:20:31	ID requested
⏵ 1	2022-03-17 18:26:25	announced

Dataset with its publication pending

submitter keyword: NSC remobilization

SnRK1

grain filling

sheath

phosphoproteomics

Oryza sativa.

Yuxiang Hu
contact affiliation	College of Agriculture, Nanjing Agricultural University, Nanjing, China
contact email	2018101028@njau.edu.cn
lab head
Hu Yuxiang
contact affiliation	College of Agriculture, Nanjing Agricultural University.
contact email	2018101028@njau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024670
     1. Label: PRIDE project
     2. Name: Quantitative proteomics reveals SnRK1-dependent protein in rice sheaths during grain filling