PXD024664

PXD024664 is an original dataset announced via ProteomeXchange.

Title	Isolation of the Buchnera aphidicola flagellum basal body complexes from the Buchnera membrane
Description	Buchnera aphidicola is an intracellular bacterial symbiont of aphids and maintains a small genome of only 600 kbps. Buchnera is thought to maintain only genes relevant to the symbiosis with its aphid host. Curiously, the Buchnera genome contains gene clusters coding for flagellum basal body structural proteins and for flagellum type III export machinery. These structures have been shown to be highly expressed and present in large numbers on Buchnera cells. No recognizable pathogenicity factors or secreted proteins have been identified in the Buchnera genome, and the relevance of this protein complex to the symbiosis is unknown. Here, we show isolation of Buchnera flagellum basal body proteins from the cellular membrane of Buchnera, confirming the enrichment of flagellum basal body proteins relative to other proteins in the Buchnera proteome. This will facilitate studies of the structure and function of the Buchnera flagellum structure, and its role in this model symbiosis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:55:53.384.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024664
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Schepers
SpeciesList	scientific name: Buchnera aphidicola str. APS (Acyrthosiphon pisum); NCBI TaxID: 107806;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:20:05	ID requested
⏵ 1	2022-02-16 16:55:54	announced

Schepers MJ, Yelland JN, Moran NA, Taylor DW, Isolation of the Buchnera aphidicola flagellum basal body complexes from the Buchnera membrane. PLoS One, 16(5):e0245710(2021) [pubmed]

submitter keyword: Buchnera, endosymbiont, flagella, basal body

Matthew Schepers
contact affiliation	Nancy Moran, Warren J. and Viola Mae Raymer Chair, Professor, The University of Texas at Austin Department of Integrative Biology. nancy.moran@austin.utexas.edu David Taylor, Lorene Morrow Kelley Endowed Faculty Fellowship Fund, Professor, The University ofTexas at Austin Department of Molecular Biosciences. david.taylor@utexas.edu
contact email	mschepers@utexas.edu
lab head
Matthew Schepers
contact affiliation	The University of Texas at Austin
contact email	mschepers@utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024664

PRIDE project URI

• PRIDE
  1. PXD024664
     1. Label: PRIDE project
     2. Name: Isolation of the Buchnera aphidicola flagellum basal body complexes from the Buchnera membrane