PXD024662

PXD024662 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Functional Inward Rectifier Potassium Channel (Kir) 2.1 for Posttranslational Modification Discovery
Description	Kir2.1 has been implicated in a number of channelopathies including Andersen-Tawil Syndrome, Short QT Syndrome and catecholaminergic polymorphic ventricular tachycardia. We developed a mass spectrometry-based assay to identify phosphorylation sites and test the functional consequence of removing those sites by site-directed mutagenesis. Our study identified novel sites of phosphorylation and suggests that the site of phosphorylation can influence channel function. We envision our approach can be readily adapted to study additional mutations and other ion channels.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_07:30:18.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kyle brown
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:19:53	ID requested
⏵ 1	2021-11-03 07:30:18	announced

Dataset with its publication pending

submitter keyword: Ion Channel, Phosphorylation, Top-down Proteomics, Bottom-up Proteomics

Ying Ge
contact affiliation	Department of Cell and Regenerative Biology, University ofWisconsin-Madison, Madison, Wisconsin, 53705, USA
contact email	ying.ge@wisc.edu
lab head
kyle brown
contact affiliation	uw madison
contact email	kbrown33@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024662

PRIDE project URI

• PRIDE
  1. PXD024662
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Functional Inward Rectifier Potassium Channel (Kir) 2.1 for Posttranslational Modification Discovery