PXD024661

PXD024661 is an original dataset announced via ProteomeXchange.

Title	A PROTAC Targets Splicing Factor 3B1
Description	The proteolysis-targeting chimera (PROTAC) is a new technology to degrade a target protein. However, its clinical application is limited currently by lack of chemical binders to target protein. For instance, it is still unknown, whether splicing factor 3B subunit 1 (SF3B1) is targetable by PROTACs. We recently identified a 2-aminothiazole derivative (herein O4I2) to promote the generation of human pluripotent stem cells. In the present work, proteomic analysis on the biotinylated O4I2 revealed that O4I2 targeted SF3B1 and positively regulated RNA splicing.
HostingRepository	PRIDE
AnnounceDate	2022-08-08
AnnouncementXML	Submission_2022-08-08_07:04:13.373.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Tascher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:19:51	ID requested
⏵ 1	2022-08-08 07:04:14	announced

Gama-Brambila RA, Chen J, Zhou J, Tascher G, M, ü, nch C, Cheng X, A PROTAC targets splicing factor 3B1. Cell Chem Biol, 28(11):1616-1627.e8(2021) [pubmed]

submitter keyword: HeLa, TMTpro, PROTAC, Orbitrap LUMOS

Xinlai Cheng
contact affiliation	Buchmann Institute for Molecular Life Sciences, Pharmaceutical Chemistry, Goethe-University Frankfurt am Main,Max-von-Laue-Strasse 15. R. 3.652, D-60438 Frankfurt amMain, Germany
contact email	cheng@pharmchem.uni-frankfurt.de
lab head
Georg Tascher
contact affiliation	Institute of Biochemistry II, Goethe University Hospital Frankfurt/Main, Germany
contact email	tascher@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD024661

PRIDE project URI

• PRIDE
  1. PXD024661
     1. Label: PRIDE project
     2. Name: A PROTAC Targets Splicing Factor 3B1