PXD024658

PXD024658 is an original dataset announced via ProteomeXchange.

Title	Proteomics investigation of the time course responses of RAW264.7 macrophages to infections with the wild-type and twin-arginine translocation mutant strains of Brucella melitensis
Description	Brucella, a notorious intracellular pathogen, causes chronic infections in many mammals, including humans. The twin-arginine translocation (Tat) pathway transports folded proteins across the cytoplasmic membrane; protein substrates translocated by Brucella include ABC transporters, oxidoreductases, and cell envelope biosynthesis proteins. Previously, we showed that a Tat mutant of Brucella melitensis M28 exhibits reduced survival within murine macrophages. In this study, we compared the host responses elicited by wild-type M28 and its Tat-mutant strains ex vivo. We utilized label-free quantitative proteomics to assess proteomic changes in RAW264.7 macrophages after infection with M28 and its Tat mutants.
HostingRepository	PRIDE
AnnounceDate	2021-07-05
AnnouncementXML	Submission_2021-07-04_21:54:49.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Yan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 02:19:32	ID requested
⏵ 1	2021-07-04 21:54:49	announced

Yan X, Hu S, Yang Y, Xu D, Liu W, Li G, Cai W, Bu Z, . Front Cell Infect Microbiol, 11():679571(2021) [pubmed]

submitter keyword: Brucella melitensis

twin-arginine protein translocation

immune response

RAW264.7 cell model

Xin Yan
contact affiliation	Harbin Veterinary Research Institute
contact email	ElijahY92@163.com
lab head
Xin Yan
contact affiliation	Harbin Veterinary Research Institute
contact email	ElijahY92@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024658

PRIDE project URI

• PRIDE
  1. PXD024658
     1. Label: PRIDE project
     2. Name: Proteomics investigation of the time course responses of RAW264.7 macrophages to infections with the wild-type and twin-arginine translocation mutant strains of Brucella melitensis