PXD024640 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | BS3 intermolecular crosslinking map of SAGA and SLIK (SAGA-like) transcription coactivators |
Description | The SAGA-like complex SLIK is a modified version of the Spt-Ada-Gcn5-Acetyltransferase (SAGA) complex. SLIK is formed through C-terminal truncation of the Spt7 SAGA subunit, causing loss of Spt8 that interacts with the TATA-binding protein. SLIK and SAGA are both coactivators of RNA polymerase II transcription in yeast. In addition, both SAGA and SLIK perform chromatin modifications and the two complexes have been speculated to uniquely contribute to transcription regulation. To test the respective contribution of SAGA vs. SLIK in transcription regulation, we assayed the chromatin modifying functions of SAGA vs. SLIK, revealing identical kinetics on minimal substrates in vitro. Furthermore, we determined a low-resolution cryo-EM structure of SLIK, revealing a modular architecture identical to SAGA. Finally, we performed a comprehensive study of DNA-binding properties of both coactivators. Purified SAGA and SLIK both associate with ssDNA and dsDNA with high affinity (KD = 10-17 nM) and the binding is sequence-independent. In conclusion, our study shows that the cleavage of Spt7 and the absence of Spt8 subunit in SLIK neither drive any major conformational differences in its structure compared to SAGA, nor significantly affect HAT, DUB or DNA binding activities in vitro. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-19 |
AnnouncementXML | Submission_2021-04-18_22:56:28.759.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ralf Schittenhelm |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-10 03:25:46 | ID requested | |
1 | 2021-04-18 18:45:35 | announced | |
⏵ 2 | 2021-04-18 22:56:29 | announced | 2021-04-19: Updated project metadata. |
Publication List
10.1016/J.JBC.2021.100671; |
Adamus K, Reboul C, Voss J, Huang C, Schittenhelm RB, Le SN, Ellisdon AM, Elmlund H, Boudes M, Elmlund D, SAGA and SAGA-like SLIK transcriptional coactivators are structurally and biochemically equivalent. J Biol Chem, 296():100671(2021) [pubmed] |
Keyword List
submitter keyword: DNA-protein interaction, Cryo-electron microscopy, Histone deubiquitination,Transcriptional coactivator, Histone acetylation |
Contact List
Marion Boudes |
contact affiliation | Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton VIC 3800, Australia |
contact email | marion.boudes@monash.edu |
lab head | |
Ralf Schittenhelm |
contact affiliation | Monash University |
contact email | ralf.schittenhelm@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024640
- Label: PRIDE project
- Name: BS3 intermolecular crosslinking map of SAGA and SLIK (SAGA-like) transcription coactivators