PXD024637

PXD024637 is an original dataset announced via ProteomeXchange.

Title	Shotgun LC-MS analysis of aorta and liver tissues from WT and Gal-1 KO mice to study the role of Galectin-1 in vascular remodeling in atherosclerosis and abdominal aortic aneurysm
Description	Pathological vascular remodeling is the underlying cause of main cardiovascular diseases (CVD) including atherosclerosis and abdominal aortic aneurysm (AAA). We analyzed the potential role of galectin-1 (Gal-1) in atherosclerosis and AAA. Atherosclerosis was induced in mice lacking Gal-1 (Lgals1-/-) and wild-type (WT) by pAAV/D377Y-mPCSK9 adenovirus injection (1011 vector genome copies) followed by western diet during 16 weeks. In a second model, atherosclerostic WT mice were treated with recombinant Gal-1 protein (rGal-1, 100 µg 3 days/week) or vehicle during 16 weeks. Moreover, the same therapeutic approach was performed in elastase-induced AAA in mice treated for 2 weeks. Finally, Gal-1 expression was assessed in human atherosclerotic plaques and AAA lesions. Gal-1 deletion led to higher atherosclerotic burden along the aorta and larger atherosclerotic plaques in the aortic root. This deleterious effect was associated to higher circulating and lesional lipid levels and lower alpha- smooth muscle actin (α-SMA) staining in the plaques. Proteomic analysis of aorta homogenates showed an upregulation of Fibronectin and VCAM-1 in Lgals1-/- mice as compared to WT aortic tissues. In vitro experiments in VSMCs from Lgals1-/- mice or transfected with Gal-1 siRNA showed increased Fibronectin, VCAM-1 and KLF4 mRNA expression along with a decrease in α-SMA mRNA expression. Treatment with rGal-1 decreased atherosclerotic plaques (size and burden) and elastase-induced aortic dilatation, associated to higher content of α-SMA staining, in both experimental models. Gal-1 protein and mRNA tissue levels were decreased in human atherosclerotic plaques and AAA as compared to control aortic tissues.
HostingRepository	PRIDE
AnnounceDate	2022-04-04
AnnouncementXML	Submission_2022-04-03_18:29:11.179.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Estefanía Núñez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-10 03:25:30	ID requested
⏵ 1	2022-04-03 18:29:11	announced

Rold, á, n-Montero R, P, é, rez-S, á, ez JM, Cerro-Pardo I, Oller J, Martinez-Lopez D, Nu, ñ, ez E, Maller SM, Gutierrez-Mu, ñ, oz C, Mendez-Barbero N, Escola-Gil JC, Michel JB, Mittelbrunn M, V, á, zquez J, Blanco-Colio LM, Rabinovich GA, Martin-Ventura JL, Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm. Sci Adv, 8(11):eabm7322(2022) [pubmed]

submitter keyword: Mouse,Liver,Aorta,LC-MS

Jesús Vázquez
contact affiliation	Laboratorio de Proteómica Cardiovascular,Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid,Spain
contact email	jvazquez@cnic.es
lab head
Estefanía Núñez
contact affiliation	Centro Nacional de Investigaciones Cardiovasculares
contact email	enunez@cnic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024637
     1. Label: PRIDE project
     2. Name: Shotgun LC-MS analysis of aorta and liver tissues from WT and Gal-1 KO mice to study the role of Galectin-1 in vascular remodeling in atherosclerosis and abdominal aortic aneurysm