PXD024637
PXD024637 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun LC-MS analysis of aorta and liver tissues from WT and Gal-1 KO mice to study the role of Galectin-1 in vascular remodeling in atherosclerosis and abdominal aortic aneurysm |
Description | Pathological vascular remodeling is the underlying cause of main cardiovascular diseases (CVD) including atherosclerosis and abdominal aortic aneurysm (AAA). We analyzed the potential role of galectin-1 (Gal-1) in atherosclerosis and AAA. Atherosclerosis was induced in mice lacking Gal-1 (Lgals1-/-) and wild-type (WT) by pAAV/D377Y-mPCSK9 adenovirus injection (1011 vector genome copies) followed by western diet during 16 weeks. In a second model, atherosclerostic WT mice were treated with recombinant Gal-1 protein (rGal-1, 100 µg 3 days/week) or vehicle during 16 weeks. Moreover, the same therapeutic approach was performed in elastase-induced AAA in mice treated for 2 weeks. Finally, Gal-1 expression was assessed in human atherosclerotic plaques and AAA lesions. Gal-1 deletion led to higher atherosclerotic burden along the aorta and larger atherosclerotic plaques in the aortic root. This deleterious effect was associated to higher circulating and lesional lipid levels and lower alpha- smooth muscle actin (α-SMA) staining in the plaques. Proteomic analysis of aorta homogenates showed an upregulation of Fibronectin and VCAM-1 in Lgals1-/- mice as compared to WT aortic tissues. In vitro experiments in VSMCs from Lgals1-/- mice or transfected with Gal-1 siRNA showed increased Fibronectin, VCAM-1 and KLF4 mRNA expression along with a decrease in α-SMA mRNA expression. Treatment with rGal-1 decreased atherosclerotic plaques (size and burden) and elastase-induced aortic dilatation, associated to higher content of α-SMA staining, in both experimental models. Gal-1 protein and mRNA tissue levels were decreased in human atherosclerotic plaques and AAA as compared to control aortic tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-04 |
AnnouncementXML | Submission_2022-04-03_18:29:11.179.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Estefanía Núñez |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-10 03:25:30 | ID requested | |
⏵ 1 | 2022-04-03 18:29:11 | announced |
Publication List
Rold, á, n-Montero R, P, é, rez-S, á, ez JM, Cerro-Pardo I, Oller J, Martinez-Lopez D, Nu, ñ, ez E, Maller SM, Gutierrez-Mu, ñ, oz C, Mendez-Barbero N, Escola-Gil JC, Michel JB, Mittelbrunn M, V, á, zquez J, Blanco-Colio LM, Rabinovich GA, Martin-Ventura JL, Galectin-1 prevents pathological vascular remodeling in atherosclerosis and abdominal aortic aneurysm. Sci Adv, 8(11):eabm7322(2022) [pubmed] |
Keyword List
submitter keyword: Mouse,Liver,Aorta,LC-MS |
Contact List
Jesús Vázquez | |
---|---|
contact affiliation | Laboratorio de Proteómica Cardiovascular,Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid,Spain |
contact email | jvazquez@cnic.es |
lab head | |
Estefanía Núñez | |
contact affiliation | Centro Nacional de Investigaciones Cardiovasculares |
contact email | enunez@cnic.es |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD024637 |
PRIDE project URI |
Repository Record List
[ + ]