PXD024636

PXD024636 is an original dataset announced via ProteomeXchange.

Title	Inhibition of cathepsin B and SAPC secreted by HIV-infected macrophages reverse common and unique apoptosis pathways
Description	Human immunodeficiency virus 1 (HIV-1) infects blood monocytes that cross the blood-brain barrier to the central nervous system inducing neuronal damage. This damage is prompted by the secretion of viral and neurotoxic factors by HIV-infected macrophages and can result in HIV associated neurocognitive disorders (HAND). One of these neurotoxic factors secreted by HIV-infected macrophages is cathepsin B (CATB), a lysosomal cysteine protease that plays an important role in neurodegeneration. CATB interacts with Serum Amyloid P component (SAPC) contributing to HIV-induced neurotoxicity. However, the neuronal apoptosis pathways triggered by CATB and SAPC remain unknown. We aimed to elucidate these pathways in neurons exposed to HIV-infected macrophage conditioned media (MCM) before and after inhibition of CATB or SAPC using Tandem Mass Tag (TMT) proteomics labeling. Based on significant fold change (FC) ≥ ǀ2ǀ and p-value < 0.05 criteria, a total of 10, 48 and 13 proteins were deregulated after inhibiting CATB, SAPC antibodies and the cathepsin B inhibitor CA-074, respectively. We found that antibodies against CATB and SAPC, as well as the CATB inhibitor CA-074 downregulated apoptosis related proteins. CATB, SAPC or apoptotic related proteins could become potential targets against HIV-induced neuronal degeneration.
HostingRepository	PRIDE
AnnounceDate	2022-01-20
AnnouncementXML	Submission_2022-01-20_10:03:38.502.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024636
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yadira Cantres
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-10 02:22:45	ID requested
1	2022-01-20 10:00:59	announced
⏵ 2	2022-01-20 10:03:39	announced	2022-01-20: Updated publication reference for PubMed record(s): 34994563.

Zen, ó, n-Mel, é, ndez CN, Carrasquillo Carri, ó, n K, Cantres Rosario Y, Roche Lima A, Mel, é, ndez LM, Inhibition of Cathepsin B and SAPC Secreted by HIV-Infected Macrophages Reverses Common and Unique Apoptosis Pathways. J Proteome Res, 21(2):301-312(2022) [pubmed]

submitter keyword: HIV-1, Cathepsin B, SAPC, HAND, Apoptosis, TMT

Loyda Melendez
contact affiliation	University of Puerto Rico, Medical Sciences Campus, Microbioly and Zoology Department, Translational Proteomics Center
contact email	loyda.melendez@upr.edu
lab head
Yadira Cantres
contact affiliation	Universtity of Puerto Rico, Comprehensive Cancer Center
contact email	yadira.cantres@upr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024636
     1. Label: PRIDE project
     2. Name: Inhibition of cathepsin B and SAPC secreted by HIV-infected macrophages reverse common and unique apoptosis pathways