PXD024629

PXD024629 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair
Description	Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of 2-D GE analysis, a total of 35 differentially regulated proteins were identified during injury in which 22 and 13 proteins were upregulated and downregulated, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in serotonin/ acetyl choline synthesis and calcium signalling, whereas downregulated proteins were majorly involving in ubiquitin mediated proteolysis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that injury initiates multiple molecular mechanisms to repair the damage.
HostingRepository	PRIDE
AnnounceDate	2021-04-16
AnnouncementXML	Submission_2021-04-15_21:53:35.198.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Krishnaswamy Balamurugan
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iodoacetamide derivatized residue
Instrument	AXIMA Performance MALDI-TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-10 02:19:59	ID requested
⏵ 1	2021-04-15 21:53:35	announced

Pooranachithra M, Satheesh Kumar C, Bhaskar JP, Venkateswaran K, Ravichandiran V, Balamurugan K, Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair. J Proteomics, 240():104222(2021) [pubmed]

submitter keyword: C. elegans, Whole body, Injury, 2-D GE

Dr. KB Lab (Host Pathogen Interaction lab)
contact affiliation	Professor
contact email	bsuryar@yahoo.com
lab head
Krishnaswamy Balamurugan
contact affiliation	Alagappa University
contact email	bsuryar@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024629
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair