PXD024629 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair |
Description | Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of 2-D GE analysis, a total of 35 differentially regulated proteins were identified during injury in which 22 and 13 proteins were upregulated and downregulated, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in serotonin/ acetyl choline synthesis and calcium signalling, whereas downregulated proteins were majorly involving in ubiquitin mediated proteolysis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that injury initiates multiple molecular mechanisms to repair the damage. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-16 |
AnnouncementXML | Submission_2021-04-15_21:53:35.198.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Krishnaswamy Balamurugan |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | iodoacetamide derivatized residue |
Instrument | AXIMA Performance MALDI-TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-10 02:19:59 | ID requested | |
⏵ 1 | 2021-04-15 21:53:35 | announced | |
Publication List
Pooranachithra M, Satheesh Kumar C, Bhaskar JP, Venkateswaran K, Ravichandiran V, Balamurugan K, Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair. J Proteomics, 240():104222(2021) [pubmed] |
Keyword List
submitter keyword: C. elegans, Whole body, Injury, 2-D GE |
Contact List
Dr. KB Lab (Host Pathogen Interaction lab) |
contact affiliation | Professor |
contact email | bsuryar@yahoo.com |
lab head | |
Krishnaswamy Balamurugan |
contact affiliation | Alagappa University |
contact email | bsuryar@yahoo.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024629
- Label: PRIDE project
- Name: Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair