PXD024617

PXD024617 is an original dataset announced via ProteomeXchange.

Title	Chronic temperature-induced liver proteome changes in two populations of threespine stickleback (Gasterosteus aculeatus) revealed by data-independent acquisition mass spectrometry
Description	A data-independent acquisition assay library was generated for the liver of threespine sticklebacks to evaluate alterations in protein abundance and functional enrichment of molecular pathways following either a chronic warm (25°C) or cold (7°C) three-week temperature challenge in two estuarine populations. The assay library was created from a data-dependent acquisition (DDA) based raw spectral library that was filtered to remove low quality or ambiguous peptides. Functional enrichment analyses using STRING aided in identifying larger networks and domains that were significantly enriched in the different groups. The Big Lagoon population (BL) had a stronger response to both temperatures than the Klamath River population (KL). At 7°C, BL showed alterations in protein homeostasis that likely fueled a higher demand for energy, but both populations appeared to successfully acclimate to this temperature. The warm temperature acclimation saw major increases in proteins involved in chromatin structure, including a variety of histones, and transcription, while there were decreases in proteins related to translation and fatty acid metabolism. Functional enrichment analyses uncovered differences in metabolism and highlighted altered molecular pathways that only had a few representative proteins reach significance from the individual quantification analyses. This data set represents the unique proteomic signatures of two populations of threespine sticklebacks and helps elucidate general themes as well as highlight population differences in acclimating to chronic temperature stress. The synchronous expression patterns of numerous proteins gives better context and specificity to a particular stressor and helps identify the strategies and mechanisms utilized to overcome changes in the environment.
HostingRepository	PanoramaPublic
AnnounceDate	2021-06-21
AnnouncementXML	Submission_2021-06-21_20:15:56.428.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bryn Levitan
SpeciesList	scientific name: Gasterosteus aculeatus; NCBI TaxID: 69293;
ModificationList	Acetyl; Oxidation; Carbamidomethyl
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 22:00:51	ID requested
⏵ 1	2021-06-21 20:15:56	announced

Dataset with its publication pending

submitter keyword: proteomics, chronic temperature stress, liver, threespine stickleback

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Bryn Levitan
contact affiliation	UC Davis
contact email	bblevitan@ucdavis.edu
dataset submitter

Panorama Public dataset URI