PXD024609

PXD024609 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS of the reaction center-light harvesting 1 complex from Gemmatimonas phototrophica
Description	Gemmatimonas phototrophica is the only phototrophic member of the recently discovered bacterial phylum Gemmatimonadetes. It was isolated from a freshwater lake in the Gobi desert and first described in 2014. So far, Gemmatimonas phototrophica is the only bacterium to have received a complete set of photosynthesis-related genes by horizontal gene transfer from an ancient phototrophic species from the phylum Proteobacteria. This organism illustrates the possibility for engineering phototrophic capability in a non-phototrophic organism and is therefore of great interest to the field of synthetic biology. The structure of the photosynthetic reaction center-light harvesting 1 complex is under investigation by cryo-EM. Proteomic analysis verified the identities of the expected protein components of this complex and, additionally revealed polypeptides that were previously undiscovered and could be mapped to the cryo-EM images.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_07:09:26.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Gemmatimonas phototrophica; NCBI TaxID: 1379270;
ModificationList	methylthiolated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 05:36:03	ID requested
⏵ 1	2022-05-25 07:09:28	announced

Qian P, Gardiner AT, Š, í, mov, á I, Naydenova K, Croll TI, Jackson PJ, Nupur, Kloz M, Č, ub, á, kov, á P, Kuzma M, Zeng Y, Castro-Hartmann P, van Knippenberg B, Goldie KN, Kaftan D, Hrouzek P, H, á, jek J, Agirre J, Siebert CA, B, í, na D, Sader K, Stahlberg H, Sobotka R, Russo CJ, Pol, í, vka T, Hunter CN, Kobl, í, ž, ek M, photosystem. Sci Adv, 8(7):eabk3139(2022) [pubmed]

submitter keyword: LC-MS/MS, Gemmatimonas phototrophica, Gemmatimonadetes, photosynthesis, reaction center light-harvesting-1 complex

Pu Qian
contact affiliation	Molecular Biology and Biotechnology, University of Sheffield, UK
contact email	p.qian@sheffield.ac.uk
lab head
Philip Jackson
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD024609

PRIDE project URI

• PRIDE
  1. PXD024609
     1. Label: PRIDE project
     2. Name: LC-MS/MS of the reaction center-light harvesting 1 complex from Gemmatimonas phototrophica