PXD024607

PXD024607 is an original dataset announced via ProteomeXchange.

Title	Discovery of Thymosin beta-4 as a human exerkine and growth factor
Description	Skeletal muscle has emerged as an endocrine organ secreting exercise-induced factors (exerkines), which play a pivotal role in inter-organ crosstalk. Via MS-based proteomics, we identified thymosin beta-4 (TMSB4X) to be the most upregulated secreted protein in the media of contracting C2C12 myotubes. TMSB4X was also acutely increased in the plasma of exercising humans and there was a net uptake of TMSB4X across the thigh during exercise. Chronic treatment of TMSB4X in mice did not ameliorate the metabolic disruptions associated with diet induced-obesity, nor did it enhance muscle regeneration in vivo. However, TMSB4X did increase osteoblast proliferation and neurite outgrowth, consistent with its WADA-classification as a prohibited growth factor. Thus, we report TMSB4X as a novel human exerkine with a potential role in cellular crosstalk.
HostingRepository	PRIDE
AnnounceDate	2021-09-15
AnnouncementXML	Submission_2021-09-15_05:52:12.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 05:32:19	ID requested
⏵ 1	2021-09-15 05:52:13	announced

Gonzalez-Franquesa A, Stocks B, Borg ML, Kuefner M, Dalbram E, Nielsen TS, Agrawal A, Pankratova S, Chibalin AV, Karlsson HKR, Gheibi S, Bj, ö, rnholm M, J, ø, rgensen NR, Clemmensen C, Hostrup M, Treebak JT, Krook A, Zierath JR, Deshmukh AS, 4 as a human exerkine and growth factor. Am J Physiol Cell Physiol, 321(5):C770-C778(2021) [pubmed]

submitter keyword: myokine, secreted factor, contraction, exercise, growth factor

Atul Shahaji Deshmukh
contact affiliation	Novo Nordisk Center for Basic Metabolic Research, University of Copenhagen, Denmark.
contact email	atul.deshmukh@sund.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024607

PRIDE project URI

• PRIDE
  1. PXD024607
     1. Label: PRIDE project
     2. Name: Discovery of Thymosin beta-4 as a human exerkine and growth factor