PXD024605

PXD024605 is an original dataset announced via ProteomeXchange.

Title	Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper
Description	Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles.
HostingRepository	PRIDE
AnnounceDate	2021-08-13
AnnouncementXML	Submission_2021-08-13_08:31:20.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Capsicum chinense x Capsicum frutescens; NCBI TaxID: 936530;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 01:30:22	ID requested
⏵ 1	2021-08-13 08:31:20	announced

Dataset with its publication pending

submitter keyword: Ghost pepper, Capsicum chinense, Capsicum frutescens, Mass spectrometry, peptidomics

Leslie M. Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States
contact email	lmhicks@email.unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024605
     1. Label: PRIDE project
     2. Name: Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper