PXD024602

PXD024602 is an original dataset announced via ProteomeXchange.

Title	LILRA3-GFP and GFP only Transfected HEK293T cells LC-MSMS
Description	The leukocyte immunoglobulin-like receptor A3 (LILRA3) is a soluble protein primarily expressed by peripheral blood monocytes and is abundant in sera of healthy donors. Extracellular LILRA3 is anti-inflammatory and displays neuro-regenerative functions in vitro. However, its intracellular expression, distribution and function(s) remain unknown. Using a combination of high-resolution confocal and super-resolution microscopy, we identified intracellular expression of native LILRA3 in the nucleus of peripheral blood monocytes and in vitro derived macrophages. This unexpected nuclear localisation of LILRA3 was confirmed in LILRA3-GFP transfected HEK293T cells. Western blot of proteins fractionated from primary macrophages and the transfected HEK293T cells confirmed nuclear localisation of the native and expressed LILRA3 proteins. Interestingly, most of the LILRA3 in the nucleus was in a monomeric form like the biologically active secreted protein, while those in the other cellular compartments were mixed monomeric, dimeric, and oligomeric forms. The predominant presence of monomeric LILRA3 in the nucleus was independently corroborated in transfected live HEK293T cells using number and molecular brightness (N&B) analysis method. Immunoprecipitation and Mass spectrometric peptide sequencing studies revealed that nuclear LILRA3 co-immunoprecipitated with several nuclear proteins involved in host protein synthesis machinery via direct interaction to a key multifunctional RNA binding protein, the Ewing sarcoma breakpoint region 1 protein (EWS).
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-08_02:23:26.723.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicodemus Tedla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 01:29:12	ID requested
⏵ 1	2021-04-08 02:23:27	announced

An H, Richardson A, Rajasekariah P, Zhong L, Fernando BSM, Macmillan A, Klotzsch E, Bryant K, Kaakoush NO, Tedla N, Nuclear Leukocyte Immunoglobulin-like Receptor A3 Is Monomeric and Is Involved in Multiple Layers of Regulated Gene Expression and Translation. J Proteome Res, 20(6):3078-3089(2021) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: LILRA3, HEK293T cells, LC-MSMS, Immunoprecipitation

Nicodemus Tedla
contact affiliation	Mechanisms of Diseases & Translational Research, School ofMedical Sciences, Department of Pathology, UNSW, Sydney, Australia
contact email	n.tedla@unsw.edu.au
lab head
Nicodemus Tedla
contact affiliation	The University of New South Wales
contact email	n.tedla@unsw.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024602
     1. Label: PRIDE project
     2. Name: LILRA3-GFP and GFP only Transfected HEK293T cells LC-MSMS