PXD024591

PXD024591 is an original dataset announced via ProteomeXchange.

Title	PGC-1alpha mediates a metabolic host defense response in human airway epithelium during rhinovirus infections
Description	Human rhinoviruses (HRV) are common cold viruses associated with exacerbations of lower airways diseases. Although viral induced epithelial damage mediates inflammation, the molecular mechanisms responsible for airway epithelial damage and dysfunction remain undefined. Using experimental HRV infection studies in humans and highly differentiated human bronchial epithelial cells grown at air-liquid interface (ALI), we examined the links between viral host defense, cellular metabolism, and epithelial barrier function. We observed that early HRV-C15 infection induced a transitory barrier-protective metabolic state characterized by glycolysis that ultimately becomes exhausted as the infection progresses and leads to cellular damage. Pharmacological promotion of glycolysis induced ROS-dependent upregulation of the mitochondrial metabolic regulator, peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha), thereby restoring epithelial barrier function, improving viral defense, and attenuating disease pathology. Therefore, PGC-1alpha regulates a metabolic pathway essential to host defense that can be therapeutically targeted to rescue airway epithelial barrier dysfunction and potentially prevent severe respiratory complications or secondary bacterial infections.
HostingRepository	PRIDE
AnnounceDate	2021-04-30
AnnouncementXML	Submission_2021-04-30_10:38:33.270.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 22:59:29	ID requested
⏵ 1	2021-04-30 10:38:33	announced

Dataset with its publication pending

submitter keyword: virus, metabolism, airway, lung

Antoine Dufour
contact affiliation	Dufour Lab, University of Calgary, department of Physiology and Pharmacology, Biochemistry and Molecular Biology
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024591
     1. Label: PRIDE project
     2. Name: PGC-1alpha mediates a metabolic host defense response in human airway epithelium during rhinovirus infections