PXD024578

PXD024578 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of the plant-pathogen interaction in resistant and susceptible peanut cultivars infected by Cylindrocladium parasiticum
Description	Background: Cylindrocladium parasiticum Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot (CBR) of peanut (Arachis hypogaea L.), has leaded to economic losses in China. Learning about how peanut responds to C. parasiticum infection will be conducive to designing strategies for CBR control. However, the response of peanut plant to C. parasiticum is poorly understood. Results: In this study, two contrasting peanut cultivars, T09 (C. parasiticum-resistant) and P562 (C. parasiticum-susceptible) were used for comparative analysis of protein profiles in the root segment of peanut plants in responses to C. parasiticum infection. Proteomic profiling identified 1647 and 391 differentially expressed proteins (DEPs) in A. hypogaea L. P562 and A. hypogaea L. T09, respectively, compared to controls. A total of 350 and 1095 DEPs were identified between A. hypogaea L. P562 and A. hypogaea L. T09 before and after 9 dpi, respectively. Functional categorization by GO annotation showed that C. parasiticum-responsive proteins were mainly involved in catalytic activity and binding. The results of KEGG pathway analysis indicated both resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence capacity. However, the MAPK signal pathway was more pronounced in resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid biosynthesis and flavonoid biosynthesis pathways in the susceptible peanut ecotype P562, while decrease in the resistant peanut ecotype T09, after 9 dpi. Additionally, there was a marked activation of brassinosteroid biosynthesis in the resistant T09, which indicated a possible involvement of activation of plant immune response in the resistant responses of peanut to C. parasiticum. Conclusions: This study provides some insights into the molecular networks involved on cellular and physiological responses to C. parasiticum infestation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:11:34.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xinyu chen
SpeciesList	scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 01:04:00	ID requested
1	2022-10-13 21:43:16	announced
⏵ 2	2023-11-14 08:11:35	announced	2023-11-14: Updated project metadata.

Chen X, Luo M, Wu W, Dong Z, Zou H, Black Rot. J Fungi (Basel), 8(8):(2022) [pubmed]

submitter keyword: peanut

Cylindrocladium parasiticum

proteome

plant defence

Zhangyong Dong
contact affiliation	Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, China
contact email	dongzhangyong@hotmail.com
lab head
xinyu chen
contact affiliation	Zhongkai University of Agriculture and Engineering
contact email	chenxinyu_xinyu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024578
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of the plant-pathogen interaction in resistant and susceptible peanut cultivars infected by Cylindrocladium parasiticum