PXD024574

PXD024574 is an original dataset announced via ProteomeXchange.

Title	Trypanosoma brucei histones are heavily modified with combinatorial post-translational modifications and mark Pol II transcription start regions with hyperacetylated H2A.
Description	Trypanosomes diverged from the main eukaryotic lineage about 600 million years ago, and display some unusual genomic and epigenetic properties that provide valuable insight into the early processes employed by eukaryotic ancestors to regulate chromatin-mediated functions. We sequenced Trypanosoma brucei core histones by high mass accuracy middle-down mass spectrometry to map core histone post-translational modifications (PTMs) and elucidate cis histone combinatorial PTMs (cPTMs). T. brucei histones are heavily modified and display intricate cPTMs patterns, with numerous hypermodified cPTMs that could contribute to the formation of non repressive euchromatic states. The T. brucei H2A C terminal tail is hyperacetylated, containing up to 5 acetylated lysine residues. MNase-ChIP-seq revealed a striking enrichment of hyperacetylated H2A at Pol II transcription start regions, and showed that H2A histones that are hyperacetylated in different combinations localised to different genomic regions, suggesting distinct epigenetic functions. Our genomics and proteomics data provide insight into the complex epigenetic mechanisms used by this parasite to regulate a genome that lacks the transcriptional control mechanisms found in higher eukaryotes. The findings further demonstrate the complexity of epigenetic mechanisms that were probably shared with the last eukaryotic common ancestor.
HostingRepository	PRIDE
AnnounceDate	2022-08-21
AnnouncementXML	Submission_2022-08-21_09:05:00.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JP Maree
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monomethylated residue; trimethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; mono N-acetylated residue; dimethylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 01:02:01	ID requested
⏵ 1	2022-08-21 09:05:00	announced

Dataset with its publication pending

submitter keyword: Trypanosoma brucei, combinatorial histone post-translational modification, transcription, epigenetics, histone hyperacetylation

Hugh George Patterton
contact affiliation	Centre for Bioinformatics and Computational Biology, University of Stellenbosch, South Africa
contact email	hpatterton@sun.ac.za
lab head
JP Maree
contact affiliation	Stellenbosch University
contact email	jpmareeac@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024574
     1. Label: PRIDE project
     2. Name: Trypanosoma brucei histones are heavily modified with combinatorial post-translational modifications and mark Pol II transcription start regions with hyperacetylated H2A.