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PXD024552

PXD024552 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents
DescriptionThe aim of the present study was to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at proteome level. The PEG-induced osmotic stress was studied in cotyledonary MDE of two genotypes: Cadeli (D), and Viking (V), which were previously reported to reveal contrasting leaf proteome responses under drought. 156 representative protein spots – obtained by 2D-DIGE – have been selected for MALDI-TOF/TOF, 63 proteins have been successfully identified and divided into 8 functional groups. Eight selected protein accumulation trends were validated by using qRT-PCR. Biomass accumulation in treated D was significantly higher (3-fold) than in V, so we can claim D as resistant to osmotic stress. Cultivar D displayed resistance strategy thanks to accumulation of proteins in energy metabolism, redox homeostasis, protein destination and signalling functional groups accompanied by high ABA and active cytokinins. In contrast, V protein profile displayed high requirements of energy (ATP) and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick down-regulation for active CK content, SA, JA, GA20. MDE proteome profile is discussed together with leaf proteome results from our previous study (Urban et al., 2017, 30:152, J. Proteomics). We propose different mechanisms to cope with osmotic stress in the genotypes studied. This proteomic study is a first step to validate MDE as a suitable model for follow-up research on characterization of new crossings, and can be used for selection of resistant genotypes.
HostingRepositoryPRIDE
AnnounceDate2021-06-14
AnnouncementXMLSubmission_2021-06-14_02:31:46.444.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSebastien Planchon
SpeciesList scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationListdihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument5800 TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-05 06:48:43ID requested
12021-06-14 02:31:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Microspore, 2D-DIGE, Osmotic stress, Brassica napus, RT-qPCR
Contact List
Mgr. Pavel Vitamvas, PhD
contact affiliationCrop Research Institute, Prague, Czechia
contact emailvitamvas@vurv.cz
lab head
Sebastien Planchon
contact affiliationLuxembourg Institute of Science and Technology
contact emailsebastien.planchon@list.lu
dataset submitter
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Dataset FTP location
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