PXD024552 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents |
Description | The aim of the present study was to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at proteome level. The PEG-induced osmotic stress was studied in cotyledonary MDE of two genotypes: Cadeli (D), and Viking (V), which were previously reported to reveal contrasting leaf proteome responses under drought. 156 representative protein spots – obtained by 2D-DIGE – have been selected for MALDI-TOF/TOF, 63 proteins have been successfully identified and divided into 8 functional groups. Eight selected protein accumulation trends were validated by using qRT-PCR. Biomass accumulation in treated D was significantly higher (3-fold) than in V, so we can claim D as resistant to osmotic stress. Cultivar D displayed resistance strategy thanks to accumulation of proteins in energy metabolism, redox homeostasis, protein destination and signalling functional groups accompanied by high ABA and active cytokinins. In contrast, V protein profile displayed high requirements of energy (ATP) and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick down-regulation for active CK content, SA, JA, GA20. MDE proteome profile is discussed together with leaf proteome results from our previous study (Urban et al., 2017, 30:152, J. Proteomics). We propose different mechanisms to cope with osmotic stress in the genotypes studied. This proteomic study is a first step to validate MDE as a suitable model for follow-up research on characterization of new crossings, and can be used for selection of resistant genotypes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-14 |
AnnouncementXML | Submission_2021-06-14_02:31:46.444.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sebastien Planchon |
SpeciesList | scientific name: Brassica napus (Rape); NCBI TaxID: 3708; |
ModificationList | dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 5800 TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-05 06:48:43 | ID requested | |
⏵ 1 | 2021-06-14 02:31:46 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Microspore, 2D-DIGE, Osmotic stress, Brassica napus, RT-qPCR |
Contact List
Mgr. Pavel Vitamvas, PhD |
contact affiliation | Crop Research Institute, Prague, Czechia |
contact email | vitamvas@vurv.cz |
lab head | |
Sebastien Planchon |
contact affiliation | Luxembourg Institute of Science and Technology |
contact email | sebastien.planchon@list.lu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024552
- Label: PRIDE project
- Name: The resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents