PXD024552

PXD024552 is an original dataset announced via ProteomeXchange.

Title	The resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents
Description	The aim of the present study was to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at proteome level. The PEG-induced osmotic stress was studied in cotyledonary MDE of two genotypes: Cadeli (D), and Viking (V), which were previously reported to reveal contrasting leaf proteome responses under drought. 156 representative protein spots – obtained by 2D-DIGE – have been selected for MALDI-TOF/TOF, 63 proteins have been successfully identified and divided into 8 functional groups. Eight selected protein accumulation trends were validated by using qRT-PCR. Biomass accumulation in treated D was significantly higher (3-fold) than in V, so we can claim D as resistant to osmotic stress. Cultivar D displayed resistance strategy thanks to accumulation of proteins in energy metabolism, redox homeostasis, protein destination and signalling functional groups accompanied by high ABA and active cytokinins. In contrast, V protein profile displayed high requirements of energy (ATP) and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick down-regulation for active CK content, SA, JA, GA20. MDE proteome profile is discussed together with leaf proteome results from our previous study (Urban et al., 2017, 30:152, J. Proteomics). We propose different mechanisms to cope with osmotic stress in the genotypes studied. This proteomic study is a first step to validate MDE as a suitable model for follow-up research on characterization of new crossings, and can be used for selection of resistant genotypes.
HostingRepository	PRIDE
AnnounceDate	2021-06-14
AnnouncementXML	Submission_2021-06-14_02:31:46.444.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-05 06:48:43	ID requested
⏵ 1	2021-06-14 02:31:46	announced

Dataset with its publication pending

submitter keyword: Microspore, 2D-DIGE, Osmotic stress, Brassica napus, RT-qPCR

Mgr. Pavel Vitamvas, PhD
contact affiliation	Crop Research Institute, Prague, Czechia
contact email	vitamvas@vurv.cz
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024552

PRIDE project URI

• PRIDE
  1. PXD024552
     1. Label: PRIDE project
     2. Name: The resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents