PXD024551

PXD024551 is an original dataset announced via ProteomeXchange.

Title	Loss of RET promotes mesenchymal identity in neuroblastoma cells
Description	Aberrant activation of Anaplastic Lymphoma Kinase (ALK) drives neuroblastoma (NB). Previous work has identified the RET receptor tyrosine kinase (RTK) as a downstream target of ALK activity in NB models. We show here that ALK activation in response to ALKAL2 ligand results in the rapid phosphorylation of RET in NB cells, providing additional insight into the contribution of RET to the ALK driven gene signature in NB. To further address the role of RET in NB, RET knock-out (KO) SK-N-AS cells were generated by CRISPR/Cas9 genome engineering. Gene expression analysis of RET KO NB cells identified a reprogramming of NB cells to a mesenchymal (MES) phenotype that was characterized by increased migration and upregulation of the AXL and MET RTKs as well as integrins and extracellular matrix components. Strikingly, the upregulation of AXL in the absence of RET reflects the development timeline observed in the neural crest as progenitor cells undergo differentiation during embryonic development. Together, these findings suggest that a MES phenotype is promoted in mesenchymal NB cells in the absence of RET, reflective of a less differentiated developmental status.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:32:54.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-05 06:48:42	ID requested
⏵ 1	2022-02-16 16:32:55	announced

Siaw JT, Gabre JL, U, ç, kun E, Vigny M, Zhang W, Van den Eynden J, Hallberg B, Palmer RH, Guan J, Loss of RET Promotes Mesenchymal Identity in Neuroblastoma Cells. Cancers (Basel), 13(8):(2021) [pubmed]

submitter keyword: ALK, ALKAL2, EMT, neural differentiation, adrenergic, retinoic acid

Ruth Palmer
contact affiliation	Dept. of Medical Biochemistry and Cell Biology Institute of Biomedicine The Sahlgrenska Academy at the University of Gothenburg SWEDEN
contact email	Ruth.palmer@gu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024551

PRIDE project URI

• PRIDE
  1. PXD024551
     1. Label: PRIDE project
     2. Name: Loss of RET promotes mesenchymal identity in neuroblastoma cells