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PXD024551

PXD024551 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLoss of RET promotes mesenchymal identity in neuroblastoma cells
DescriptionAberrant activation of Anaplastic Lymphoma Kinase (ALK) drives neuroblastoma (NB). Previous work has identified the RET receptor tyrosine kinase (RTK) as a downstream target of ALK activity in NB models. We show here that ALK activation in response to ALKAL2 ligand results in the rapid phosphorylation of RET in NB cells, providing additional insight into the contribution of RET to the ALK driven gene signature in NB. To further address the role of RET in NB, RET knock-out (KO) SK-N-AS cells were generated by CRISPR/Cas9 genome engineering. Gene expression analysis of RET KO NB cells identified a reprogramming of NB cells to a mesenchymal (MES) phenotype that was characterized by increased migration and upregulation of the AXL and MET RTKs as well as integrins and extracellular matrix components. Strikingly, the upregulation of AXL in the absence of RET reflects the development timeline observed in the neural crest as progenitor cells undergo differentiation during embryonic development. Together, these findings suggest that a MES phenotype is promoted in mesenchymal NB cells in the absence of RET, reflective of a less differentiated developmental status.
HostingRepositoryPRIDE
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2022-02-16_16:32:54.255.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterProteomics Core Facility
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-05 06:48:42ID requested
12022-02-16 16:32:55announced
Publication List
Siaw JT, Gabre JL, U, รง, kun E, Vigny M, Zhang W, Van den Eynden J, Hallberg B, Palmer RH, Guan J, Loss of RET Promotes Mesenchymal Identity in Neuroblastoma Cells. Cancers (Basel), 13(8):(2021) [pubmed]
Keyword List
submitter keyword: ALK, ALKAL2, EMT, neural differentiation, adrenergic, retinoic acid
Contact List
Ruth Palmer
contact affiliationDept. of Medical Biochemistry and Cell Biology Institute of Biomedicine The Sahlgrenska Academy at the University of Gothenburg SWEDEN
contact emailRuth.palmer@gu.se
lab head
Proteomics Core Facility
contact affiliationSAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact emailgupcf@outlook.com
dataset submitter
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Dataset FTP location
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