PXD024551 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Loss of RET promotes mesenchymal identity in neuroblastoma cells |
Description | Aberrant activation of Anaplastic Lymphoma Kinase (ALK) drives neuroblastoma (NB). Previous work has identified the RET receptor tyrosine kinase (RTK) as a downstream target of ALK activity in NB models. We show here that ALK activation in response to ALKAL2 ligand results in the rapid phosphorylation of RET in NB cells, providing additional insight into the contribution of RET to the ALK driven gene signature in NB. To further address the role of RET in NB, RET knock-out (KO) SK-N-AS cells were generated by CRISPR/Cas9 genome engineering. Gene expression analysis of RET KO NB cells identified a reprogramming of NB cells to a mesenchymal (MES) phenotype that was characterized by increased migration and upregulation of the AXL and MET RTKs as well as integrins and extracellular matrix components. Strikingly, the upregulation of AXL in the absence of RET reflects the development timeline observed in the neural crest as progenitor cells undergo differentiation during embryonic development. Together, these findings suggest that a MES phenotype is promoted in mesenchymal NB cells in the absence of RET, reflective of a less differentiated developmental status. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-16_16:32:54.255.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Proteomics Core Facility |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-05 06:48:42 | ID requested | |
⏵ 1 | 2022-02-16 16:32:55 | announced | |
Publication List
Siaw JT, Gabre JL, U, รง, kun E, Vigny M, Zhang W, Van den Eynden J, Hallberg B, Palmer RH, Guan J, Loss of RET Promotes Mesenchymal Identity in Neuroblastoma Cells. Cancers (Basel), 13(8):(2021) [pubmed] |
Keyword List
submitter keyword: ALK, ALKAL2, EMT, neural differentiation, adrenergic, retinoic acid |
Contact List
Ruth Palmer |
contact affiliation | Dept. of Medical Biochemistry and Cell Biology Institute of Biomedicine The Sahlgrenska Academy at the University of Gothenburg SWEDEN |
contact email | Ruth.palmer@gu.se |
lab head | |
Proteomics Core Facility |
contact affiliation | SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg |
contact email | gupcf@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024551
- Label: PRIDE project
- Name: Loss of RET promotes mesenchymal identity in neuroblastoma cells