PXD024546

PXD024546 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of polysomes isolated from human cells infected with polio, zika or dengue viruses
Description	Viral pathogens are an ongoing threat to public health worldwide. Dissecting their dependence on host biosynthetic pathways could lead to effective antiviral therapies. To define how entero- and flaviviruses redirect host ribosomes to synthesize viral proteins and disable host protein production, we performed proteomic analysis of lysates and isolated polysomes from human Huh7 cells infected with either polio, zika or dengue viruses. We find that infection remodels polysome composition along similar principles, without major changes to core ribosome stoichiometry. These viruses use different strategies to evictfrom polysomes a common set of translation initiation and RNA surveillance factors while recruiting host machineries specifically required for viral biogenesis. We also find that both zika and dengue utilize the collagen prolyl-hydroxylation machinery to mediate co-translational modification of conserved prolines in the viral polyprotein. Our findings show how RNA viruses co-opt polysome modularity and establish a powerful strategy to identify targets for selective antiviral interventions.
HostingRepository	PRIDE
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-09-15_23:19:01.472.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ranen Aviner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-05 03:37:58	ID requested
1	2021-08-17 02:22:36	announced
⏵ 2	2021-09-15 23:19:02	announced	2021-09-16: Updated publication reference for PubMed record(s): 34408324.

Aviner R, Li KH, Frydman J, Andino R, Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis. Nature, 596(7873):558-564(2021) [pubmed]

submitter keyword: LC-MS/MS, ribosomes, polysomes, human, viruses, infection, translation, protein synthesis, protein folding, proteostasis

Raul Andino
contact affiliation	Department of Microbiology and Immunology University of California, San Francisco (UCSF) USA
contact email	raul.andino@ucsf.edu
lab head
Ranen Aviner
contact affiliation	Stanford University
contact email	ranen.aviner@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024546

PRIDE project URI

• PRIDE
  1. PXD024546
     1. Label: PRIDE project
     2. Name: Proteomic analysis of polysomes isolated from human cells infected with polio, zika or dengue viruses