PXD024532

PXD024532 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis
Description	The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.
HostingRepository	MassIVE
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2021-04-06_09:10:50.748.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Adamo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Phospho
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-04 16:38:03	ID requested
⏵ 1	2021-04-06 09:10:51	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: shugoshin, centromere, cohesin, mitosis, meiosis, hSgo1, PP2A-B56, coiled coil, cohesion, PP2A, B56, Cdk1

submitter keyword: shugoshin, centromere, cohesin, mitosis, meiosis, hSgo1, PP2A-B56, coiled coil, cohesion, PP2A, B56, Cdk1

Contact List

Arminja Kettenbach
contact affiliation	The Geisel School of Medicine at Dartmouth
contact email	arminja.n.kettenbach@dartmouth.edu
lab head
Mark Adamo
contact affiliation	Dartmouth College
contact email	mark.e.adamo@dartmouth.edu
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087003/