PXD024526

PXD024526 is an original dataset announced via ProteomeXchange.

Title	Proteins identified by MS on liver lysates immunoprecipitated with an antibody against O-GlcNAcylated proteins (RL2).
Description	Protein O-GlcNAcylation is a fast-cycling and nutrient-sensitive post-translational modification (PTM) involved in several functions, including hepatic metabolsm. The substrate of O-GlcNAcylation is UDP-GlcNAc, the end-product of the hexosamine biosynthesis pathway that is initiated by glutamine. O-GlcNAcylation is known to regulate several biochemical processes by modulation of the enzyme activity among other functions, we hypothesized that O-GlcNAcylation regulates the enzyme activity of one or more urea cycle enzymes, a key pathway for waste nitrogen detoxification. To test this notion, we performed immunoprecipitation of male wild-type (C57BL/6) mouse liver lysates with a well-established anti-O-GlcNAc antibody (RL2) followed by mass spectrometry analysis in order to identify proteins undergo this PTM.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:15.142.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leandro R. Soria
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-04 07:32:52	ID requested
1	2022-09-06 00:35:52	announced
⏵ 2	2023-11-14 08:52:15	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: liver,O-GlcNAcylation

OGT

OGA, mouse

Nicola Brunetti-Pierri
contact affiliation	Telethon Institute of Genetics and Medicine, Pozzuoli, Italy
contact email	brunetti@tigem.it
lab head
Leandro R. Soria
contact affiliation	TIGEM
contact email	l.soria@tigem.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD024526

PRIDE project URI

• PRIDE
  1. PXD024526
     1. Label: PRIDE project
     2. Name: Proteins identified by MS on liver lysates immunoprecipitated with an antibody against O-GlcNAcylated proteins (RL2).