PXD024522

PXD024522 is an original dataset announced via ProteomeXchange.

Title	Proteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet
Description	Pancreatic islets are essential for maintaining physiological blood glucose levels, and declining islet function is a hallmark of type 2 diabetes. Here we employ mass spectrometry (MS)-based proteomics to systematically analyse purified islets from 9 genetic or diet-induced mouse models representing a broad cross-section of metabolic health. Quantifying the islet proteome to a depth of >11,500 proteins, this study represents the most detailed analysis of islet proteins to date. Our data highlight that the vast majority of islet proteins are expressed in all strains and diets, but more than half of the proteins vary in expression levels. Associating these varied protein expression levels on an individual animal basis with individual phenotypic measures reveals islet mitochondrial function as a major positive indicator of metabolic health. This compendium of strain-specific and dietary changes to mouse islet proteomes represents a comprehensive resource for basic and translational islet cell biology.
HostingRepository	PRIDE
AnnounceDate	2021-10-08
AnnouncementXML	Submission_2021-10-08_03:10:36.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Humphrey
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-04 04:30:48	ID requested
⏵ 1	2021-10-08 03:10:36	announced

Dataset with its publication pending

submitter keyword: islets, pancreas, beta cells, alpha cells, proteome, library

Sean Humphrey
contact affiliation	School of Life and Environmental Sciences, Charles Perkins Centre, The University of Sydney
contact email	sean.humphrey@sydney.edu.au
lab head
Sean Humphrey
contact affiliation	The University of Sydney
contact email	sean.j.humphrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD024522

PRIDE project URI

• PRIDE
  1. PXD024522
     1. Label: PRIDE project
     2. Name: Proteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet