PXD024519

PXD024519 is an original dataset announced via ProteomeXchange.

Title	XL-MS analysis of human tyrosine hydroxylase
Description	Tyrosine hydroxylase (TH) is a highly regulated enzyme that catalyses the rate-limiting step in the biosynthesis of dopamine (DA) and other catecholamines. Mutations and dysfunction in this enzyme lead to DA deficiency and parkinsonisms of different severity. An understanding of TH deficiency at the level of structure and stability has been lacking to date, as only structures of truncated TH forms have been available. Here, we used cryoEM and XL-MS to determine the high-resolution structure of full-length human tetrameric TH in the absence and presence of the end-product and feedback inhibitor DA bound to the active site
HostingRepository	PRIDE
AnnounceDate	2021-12-22
AnnouncementXML	Submission_2021-12-22_02:31:16.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Marcilla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-04 03:58:14	ID requested
⏵ 1	2021-12-22 02:31:17	announced

Lorente E, Marcilla M, de la Sota PG, Quijada-Freire A, Mir C, L, ó, pez D, Acid Stripping after Infection Improves the Detection of Viral HLA Class I Natural Ligands Identified by Mass Spectrometry. Int J Mol Sci, 22(19):(2021) [pubmed]

submitter keyword: XL-MS, BS3, Tyrosine hydroxylase, Dopamine

Fernando Corrales
contact affiliation	Proteomics Unit. Spanish National Biotechnology Centre. CNB-CSIC
contact email	fcorrales@cnb.csic.es
lab head
Miguel Marcilla
contact affiliation	Proteomics Unit - Dpt. of Macromolecular Structures - Spanish National Biotechnolgy Centre (CNB-CSIC)
contact email	mmarcilla@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD024519

PRIDE project URI

• PRIDE
  1. PXD024519
     1. Label: PRIDE project
     2. Name: XL-MS analysis of human tyrosine hydroxylase