PXD024504
PXD024504 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global coordination of metabolic pathways in Escherichia coli by active and passive regulation |
| Description | In this study, we identify the regulatory mechanisms that coordinate catabolism and anabolism in the bacterium Escherichia coli. Integrating protein, metabolite, and metabolic flux changes in genetically implemented gradual catabolic or anabolic limitations, we show that a combination of global and local mechanisms coordinates the response to metabolic limitations. To allocate proteomic resources between catabolism and anabolism, E. coli uses a simple global gene regulatory program. Surprisingly, this program is largely implemented by a single transcription factor Crp, which not only directly activates the expression of catabolic enzymes, but also indirectly reduces the expression of anabolic enzymes by passively sequestering cellular resources needed for their synthesis. However, this regulatory program rarely controls metabolic fluxes alone; instead, fluxes are further adjusted locally through passive changes in metabolite concentrations. Together, these mechanisms constitute a limited but effective global regulatory program that coarsely partitions resources between different parts of metabolism while ensuring the robust coordination of individual metabolic reactions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-03-10 |
| AnnouncementXML | Submission_2021-03-10_09:20:23.725.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Vadim Patsalo |
| SpeciesList | scientific name: Escherichia coli K-12; NCBI TaxID: 83333; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-03-03 11:39:06 | ID requested | |
| ⏵ 1 | 2021-03-10 09:20:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cAMP, gene expression, growth laws, regulation, cyclic AMP, gene regulation, transcription |
Contact List
| Karl Kochanowski | |
|---|---|
| contact affiliation | ETH Zurich |
| contact email | karl.kochanowski@gmail.com |
| lab head | |
| James R. Williamson | |
| contact affiliation | The Scripps Research Institute |
| contact email | jrwill@scripps.edu |
| lab head | |
| Uwe Sauer | |
| contact affiliation | ETH Zurich |
| contact email | sauer@imsb.biol.ethz.ch |
| lab head | |
| Terence Hwa | |
| contact affiliation | UC San Diego |
| contact email | hwa@ucsd.edu |
| lab head | |
| Vadim Patsalo | |
| contact affiliation | Scripps Research |
| contact email | patsalov@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086992/ |
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