PXD024501

PXD024501 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis after neuroprotective MyD88 inhibition in the retinal degeneration 10 mouse
Description	Progressive dysfunction and death of photoreceptors occurs in blinding diseases such as age-related macular degeneration and retinitis pigmentosa. The MyD88 protein is a central adaptor molecule for innate immune system Toll-like receptors (TLR) and interleukin-1 receptor (IL-1R), which are active in retinal disease and induce cytokine secretion from inflammatory cells. We recently demonstrated that inhibiting MyD88 in mouse models of retinal degeneration led to increased rod photoreceptor survival, which was associated with altered cytokine expression and increased neuroprotective microglia. However, additional molecular changes associated with MyD88 inhibitor-mediated neuroprotection are not known. Quantitative proteomics using iTRAQ LC-MS/MS is a high-throughput method ideal for providing new information about the molecular mechanisms contributing to photoreceptor protection. In this study, we used isobaric tags for relative and absolute quantification (iTRAQ) labeling followed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) for quantitative proteomic analysis on the rd10 mouse model of retinal degeneration to identify protein pathways changed by MyD88 inhibition. A total of 42 proteins were differentially expressed in retinas from mice treated with MyD88 inhibitor compared with control. Notably, increased expression of multiple crystallins and chaperones that respond to cellular stress and have anti-apoptotic properties were identified in the MyD88 inhibited mice. Additional differentially expressed biological processes included pyrophosphatase activity and peptide biosynthesis. These data suggest that inhibiting MyD88 may enhance chaperone-mediated tissue protective pathways. Therefore, this study provides new insight into molecular events that contribute to photoreceptor protection from modulating inflammation.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:26:07.871.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024501
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sanjoy Bhattacharya
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-03 08:38:32	ID requested
⏵ 1	2022-02-16 16:26:08	announced

Carmy-Bennun T, Myer C, Bhattacharya SK, Hackam AS, Quantitative proteomic analysis after neuroprotective MyD88 inhibition in the retinal degeneration 10 mouse. J Cell Mol Med, 25(20):9533-9542(2021) [pubmed]

submitter keyword: Toll-like receptors, MyD88, photoreceptors, retinal degeneration, proteomics, iTRAQ, crystallin

Sanjoy K. Bhattacharya
contact affiliation	Bascom Palmer Eye Institute University of Miami
contact email	sbhattacharya@med.miami.edu
lab head
Sanjoy Bhattacharya
contact affiliation	Bascom Palmer Eye Institute, Department of Ophthalmology, Miller School of Medicine at University of Miami, Miami, FL 33136, USA
contact email	sbhattacharya@med.miami.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024501
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis after neuroprotective MyD88 inhibition in the retinal degeneration 10 mouse