PXD024481

PXD024481 is an original dataset announced via ProteomeXchange.

Title	Tripartite separation of glomerular proteomes from reporter-free mice
Description	Renal blood filtration occurs in a functional unit called the glomerulus. The resident cell types comprise the filtration barrier, namely podocytes, mesangial cells and glomerular endothelial cells. Here we introduce a glomerular cell isolation protocol, which enables the separation of these three cell types in sufficient amounts and purity to allow detailed protein-biochemical investigations. We demonstrate that the expression of fluorescent transgenes in glomerular cells can result in proteome artifacts. We show that different mouse strains have different glomerular cell type proteomes. Further, we demonstrate the power of the technique to identify new glomerular cell type-enriched proteins and demonstrate the potential of this globally applicable technique in the dissection of cell-specific disease responses and intra-glomerular cell-type crosstalk.
HostingRepository	PRIDE
AnnounceDate	2021-11-12
AnnouncementXML	Submission_2021-11-12_05:44:28.418.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024481
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fatih Demir
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-02 23:04:06	ID requested
⏵ 1	2021-11-12 05:44:29	announced

Hatje FA, Wedekind U, Sachs W, Loreth D, Reichelt J, Demir F, Kosub C, Heintz L, Tomas NM, Huber TB, Skuza S, Sachs M, Zielinski S, Rinschen MM, Meyer-Schwesinger C, Tripartite Separation of Glomerular Cell Types and Proteomes from Reporter-Free Mice. J Am Soc Nephrol, 32(9):2175-2193(2021) [pubmed]

submitter keyword: Mouse Kidney Glomerulus Endotheliall Mesangial

Markus Matthias Rinschen
contact affiliation	Aarhus University Department of Biomedicine 8000 Aarhus C Denmark
contact email	rinschen@biomed.au.dk
lab head
Fatih Demir
contact affiliation	Aarhus University Department of Biomedicine Markus Rinschen Lab
contact email	fatih.demir@biomed.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024481

PRIDE project URI

• PRIDE
  1. PXD024481
     1. Label: PRIDE project
     2. Name: Tripartite separation of glomerular proteomes from reporter-free mice