PXD024461

PXD024461 is an original dataset announced via ProteomeXchange.

Title	Proteomics on pancreas-on-chip model
Description	In this study, we engineered a micro-well duct-on-chip platform to generate defined 3D aggregates from hiPSC-derived PPs and subsequently induce differentiation toward PDLOs. Time-resolved scRNA-seq combined with cleared immunofluorescence imaging provided a deep understanding of in vitro ductal cell type differentiation. By defining the emergent cell types at each stage of differentiation based on their gene expression profiles and organoid structures, we provide a precise cell-by-cell description of the in vitro differentiation trajectory. Transcriptional data of PDLOs were complemented by their proteome and secretome data, allowing the identification and validation of prognostic cancer marker. Thus, we show the applicability of hiPSC-derived PDLOs-on-chip for future ductal disease modeling.
HostingRepository	PRIDE
AnnounceDate	2021-07-08
AnnouncementXML	Submission_2021-07-07_21:30:32.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine von Toerne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-02 07:35:19	ID requested
⏵ 1	2021-07-07 21:30:32	announced

Dataset with its publication pending

submitter keyword: Pancreas, proteomics, secretome

Matthias Meier
contact affiliation	Helmholtz Pioneer Campus, Helmholtz Zentrum München, Munich, Germany and Medical Faculty, Technical University of Munich, Munich, Germany
contact email	matthias.meier@helmholtz-muenchen.de
lab head
Christine von Toerne
contact affiliation	Helmholtz Zentrum München
contact email	vontoerne@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024461

PRIDE project URI

• PRIDE
  1. PXD024461
     1. Label: PRIDE project
     2. Name: Proteomics on pancreas-on-chip model