PXD024445

PXD024445 is an original dataset announced via ProteomeXchange.

Title	A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects
Description	The overall goal of this study was to determine if Aquamin®, a multi-mineral natural product, would alter the expression of proteins involved in growth-regulation and differentiation in the colon. Thirty healthy human subjects (at risk for colorectal cancer) were enrolled in a three-arm, 90-day interventional trial in which Aquamin® was compared to calcium alone and placebo. Before and after the interventional period colonic biopsies were obtained. Biopsies were evalu-ated by immunohistology for expression of Ki67 (proliferation marker) and for CK20 and p21 (differentiation markers). Tandem mass tag-mass spectrometry-based detection was used to as-sess levels of multiple proteins. As compared to placebo or calcium, Aquamin® reduced the level of Ki67 expression. Neither intervention altered CK20 expression, while a trend toward in-creased p21 was observed with calcium and Aquamin®. In the proteomic screen, Aquamin® treatment resulted in many more proteins being upregulated (including cytokeratins, cell-cell adhesion molecules and components of the basement membrane) or downregulated (prolifera-tion and nucleic acid metabolism) than placebo. Calcium alone also altered the expression of many of the same proteins but not to the same extent as Aquamin®. We conclude that daily Aquamin® ingestion alters protein expression profile in the colon that could be beneficial to colonic health.
HostingRepository	PRIDE
AnnounceDate	2021-03-19
AnnouncementXML	Submission_2021-04-06_23:22:32.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Aslam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 08:07:39	ID requested
1	2021-03-19 06:18:29	announced
⏵ 2	2021-04-06 23:22:36	announced	2021-04-07: Updated publication reference for PubMed record(s): 33799486.

Aslam MN, McClintock SD, Jawad-Makki MAH, Knuver K, Ahmad HM, Basrur V, Bergin IL, Zick SM, Sen A, Turgeon DK, Varani J, A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects. Nutrients, 13(3):(2021) [pubmed]

submitter keyword: Aquamin

biomarkers

calcium

colon cancer chemoprevention

minerals

proteomic analysis

trace elements

Muhammad N. Aslam
contact affiliation	Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, USA
contact email	mnaslam@med.umich.edu
lab head
Muhammad Aslam
contact affiliation	University of Michigan
contact email	mnaslam@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024445

PRIDE project URI

• PRIDE
  1. PXD024445
     1. Label: PRIDE project
     2. Name: A Multi-Mineral Intervention to Modulate Colonic Mucosal Protein Profile: Results from a 90-Day Trial in Human Subjects