PXD024442

PXD024442 is an original dataset announced via ProteomeXchange.

Title	Regulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues
Description	For the identification of HTRA2-specific protein interaction partners in retinal tissues of the house swine (Sus scrofa), we performed co-immunoprecipitation experiments with recombinant 6His-tag HTRA2 followed by quantitative LC-MS-based proteomics (see Fig. 2A and File S1). In brief, 2 µg recombinant 6His-tag HTRA2 (HTRA2 group) were spiked into 5 mg homogenized porcine retina and was subsequently enriched with the bounded protein interaction partners via Ni-NTA affinity purification (n=3). In addition, two further sample groups were treated with 10 µg UCF 101 (UCF-101 group) or 10 µg synthetic CDR peptide (CDR group) to unravel the influence of both molecules on the HTRA2-specific protein interactome in the retinal tissues (n=3 for each group). Eluate fractions of each group were separated by 1-D SDS PAGE (Fig. S1) and subsequently subjected to in-gel trypsin digestion. To distinguish unspecific from HTRA2 specific binders, we also included a control Ni-NTA bead group (CTRL group) for the quantitative MS analysis (n=3).
HostingRepository	PRIDE
AnnounceDate	2021-10-11
AnnouncementXML	Submission_2021-10-10_23:29:50.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carsten Schmelter
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 06:18:25	ID requested
⏵ 1	2021-10-10 23:29:51	announced

Schmelter C, Fomo KN, Perumal N, Pfeiffer N, Grus FH, Regulation of the HTRA2 Protease Activity by an Inhibitory Antibody-Derived Peptide Ligand and the Influence on HTRA2-Specific Protein Interaction Networks in Retinal Tissues. Biomedicines, 9(8):(2021) [pubmed]

submitter keyword: HTRA2

Neuroprotection

Retina

Interaction partners

Co-immunoprecipitation

Mass spectrometry

Dr. Carsten Schmelter
contact affiliation	Translational and Experimental Ophthalmology, University Medical Center in Mainz
contact email	cschmelter@eye-research.org
lab head
Carsten Schmelter
contact affiliation	Universitätsmedizin Mainz
contact email	cschmelter@eye-research.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024442
     1. Label: PRIDE project
     2. Name: Regulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues