PXD024425 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | putative K29-linked substrates identification using sAB-K29 |
Description | We have identified K29-linked ubiquitination is involved in cell cycle regulation and enriched in midbody formation. To identify the substrates of K29-linked ubiquitination in the midbody, we soughtto perform immunoprecipitation (IP) under denaturing conditions to minimize the presence of noncovalently bound proteins. we synchronized HeLa cells to telophase to maximize the abundance of the midbody and lysed the cells using 8 M urea. The lysate was further diluted in 1 M urea, followed by IP using biotinylated sAB-K29. The enriched proteins were separated on an SDS-PAGE gel and subjected to label-free quantitative mass spectrometry analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-12 |
AnnouncementXML | Submission_2021-07-11_22:13:35.458.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qingyun Zheng |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-01 02:37:57 | ID requested | |
1 | 2021-07-11 04:07:13 | announced | |
⏵ 2 | 2021-07-11 22:13:36 | announced | 2021-07-12: Updated project metadata. |
Publication List
Yu Y, Zheng Q, Erramilli SK, Pan M, Park S, Xie Y, Li J, Fei J, Kossiakoff AA, Liu L, Zhao M, K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle. Nat Chem Biol, 17(8):896-905(2021) [pubmed] |
10.1038/S41589-021-00823-5; |
Keyword List
submitter keyword: K29-linked ubiquitination |
Contact List
Lei Liu |
contact affiliation | Tsinghua university, Department of chemistry |
contact email | lliu@tsinghua.edu.cn |
lab head | |
Qingyun Zheng |
contact affiliation | Department of Chemistry, Tsinghua University |
contact email | zhengqytsinghua@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024425
- Label: PRIDE project
- Name: putative K29-linked substrates identification using sAB-K29