PXD024422

PXD024422 is an original dataset announced via ProteomeXchange.

Title	Identification of Altered Proteins in the Plasma of Rats with Chronic Prostatic Inflammation Induced by Estradiol Benzoate and Sex Hormones
Description	The cause of non-bacterial chronic prostatitis is unknown, yet its prevalence accounts for more than 90% of all prostatitis cases. Whole blood, plasma, and serum have been used to identify prostate cancer biomarkers; however, few studies have performed protein profiling to identify prostatitis biomarkers. The purpose of this study was to identify protein biomarkers altered by chronic prostatitis. To perform the study, we chemically induced chronic prostate inflammation in Sprague Dawley rats using estradiol benzoate (EB), testosterone (T), and estradiol (E), then examined protein levels in their plasma. Plasma was collected on postnatal days (PNDs) 90, 100, 145, and 200; plasma proteins were profiled using liquid chromatography-tandem mass spectrometry. Chronic inflammation was observed in rat prostate induced with EB on PNDs 1, 3, and 5. Rats then were dosed with T+E during PND 90-200 via subcutaneous implants. We identified time-specific expression for several proteins (i.e., CFB, MYH9, AZGP1). Some altered proteins that were expressed in the prostate (i.e., SERPINF1, CTR9) also were identified in rat plasma in the EB+T+E group on PNDs 145 and 200. These findings suggest that the identified proteins could be used as biomarkers of chronic prostatitis. Further studies are needed to verify the results in human samples.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:16:00.938.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noriko Nakamura
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 02:32:33	ID requested
⏵ 1	2022-02-16 16:16:01	announced

Cao Z, Sloper DT, Nakamura N, Identification of Altered Proteins in the Plasma of Rats With Chronic Prostatic Inflammation Induced by Estradiol Benzoate and Sex Hormones. ACS Omega, 6(22):14361-14370(2021) [pubmed]

submitter keyword: rats, plasma, proteomics, estradiol, testosterone, estradiol benzoate, prostate, chronic inflammation

Noriko Nakamura
contact affiliation	Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
contact email	Noriko.Nakamura@fda.hhs.gov
lab head
Noriko Nakamura
contact affiliation	NCTR/FDA
contact email	Noriko.Nakamura@fda.hhs.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024422
     1. Label: PRIDE project
     2. Name: Identification of Altered Proteins in the Plasma of Rats with Chronic Prostatic Inflammation Induced by Estradiol Benzoate and Sex Hormones