PXD024417

PXD024417 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidome of HeLa-CIITA cells silenced for TAX1BP1(T6BP) expression
Description	CD4+ T lymphocytes play a major role in the establishment and maintenance of immunity. They are activated by antigenic peptides derived from extracellular or newly synthesized (endogenous) proteins presented on the surface of antigen presenting cells (APCs) by the MHC-II molecules. The pathways leading to endogenous MHC-II presentation remain poorly characterized. We demonstrate here that the autophagy receptor, T6BP, influences both autophagy-dependent and -independent endogenous presentation of HIV- and HCMV-derived peptides. By studying the immunopeptidome of MHC-II molecules, we show that T6BP affects both the quantity and quality of peptides presented. T6BP silencing induces mislocalization of the MHC-II-loading compartments and a rapid degradation of the invariant chain (CD74) without altering the expression and internalization kinetics of MHC-II molecules. T6BP also controls the ER localization of the chaperone calnexin that we identified as a T6BP partner. Remarkably, calnexin silencing in APCs replicates the functional consequences of T6BP silencing: decreased CD4+ T cell activation and exacerbated CD74 degradation. Altogether, we unravel T6BP as a key player of the MHC-II-restricted endogenous presentation pathway and we propose one potential mechanism of action.
HostingRepository	PRIDE
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-22_00:25:50.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arnaud Moris
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-28 22:59:53	ID requested
⏵ 1	2022-09-22 00:25:51	announced

Dataset with its publication pending

submitter keyword: Human, autophagy receptor, LC-MSMS

Arnaud Moris
contact affiliation	Institute for Integrative Biology of the Cell UMR 9198 CNRS, CEA, Université Paris-Saclay 1 Avenue de la Terrasse, 91190 Gif-sur-Yvette, France
contact email	arnaud.moris@i2bc.paris-saclay.fr
lab head
Arnaud Moris
contact affiliation	Institute for Integrative Biology of the Cell CNRS, CEA, Université Paris-Saclay
contact email	arnaud.moris@i2bc.paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD024417

PRIDE project URI

• PRIDE
  1. PXD024417
     1. Label: PRIDE project
     2. Name: Immunopeptidome of HeLa-CIITA cells silenced for TAX1BP1(T6BP) expression