PXD024397

PXD024397 is an original dataset announced via ProteomeXchange.

Title	Palaeoproteomic identification of binders in a 15th-century panel painting by Sandro Botticelli’s workshop
Description	The coupling of molecular analyses with the conservation of artworks has gained growing importance over the last decades, and today it can take advantage of state-of-the-art analytical techniques, such as mass spectrometry-based proteomics. This technique allows for the deep characterisation of proteins with high accuracy and reliability. Protein-based binders are among the most common organic artistic materials, and have been used in art production for centuries. However, the applications of proteomic techniques to these materials are still limited. In this work, a palaeoproteomic workflow was successfully tested on a set of paint reconstructions, and subsequently applied to micro-samples from a 15th-century panel painting, attributed to Sandro Botticelli’s workshop. The method allowed for confident identification of the protein-based binders and their biological origin in all historical micro-samples, as well as for the discrimination of the binder used in the ground and paint layers. These results were discussed thoroughly in order to outline a set of guidelines, covering the main steps of the data analysis of protein sequencing results from an artwork. This work is the first application of the adopted palaeoproteomic protocol to pigmented materials, with results showing that the approach is accurate, highly sensitive, and broadly applicable in the cultural heritage field, due to the limited amount of starting material it requires.
HostingRepository	PRIDE
AnnounceDate	2022-07-05
AnnouncementXML	Submission_2022-07-05_05:21:38.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabiana Di Gianvincenzo
SpeciesList	scientific name: Capra hircus; NCBI TaxID: 9925; scientific name: Oryctolagus cuniculus; NCBI TaxID: 9986; scientific name: Ovis aries; NCBI TaxID: 9940; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-26 04:28:05	ID requested
⏵ 1	2022-07-05 05:21:38	announced

Di Gianvincenzo F, Peggie D, Mackie M, Granzotto C, Higgitt C, Cappellini E, Palaeoproteomics guidelines to identify proteinaceous binders in artworks following the study of a 15th-century painting by Sandro Botticelli's workshop. Sci Rep, 12(1):10638(2022) [pubmed]

submitter keyword: palaeoproteomics, mass spectrometry, paint binders, cultural heritage, LC-MSMS

Enrico Cappellini
contact affiliation	Section for EvoGenomics, GLOBE Institute, University of Copenhagen, Denmark
contact email	ecappellini@sund.ku.dk
lab head
Fabiana Di Gianvincenzo
contact affiliation	EvoGenomics Section, GLOBE Institute, University of Copenhagen
contact email	fdigianvincenzo@palaeome.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024397
     1. Label: PRIDE project
     2. Name: Palaeoproteomic identification of binders in a 15th-century panel painting by Sandro Botticelli’s workshop