PXD024394

PXD024394 is an original dataset announced via ProteomeXchange.

Title	Online Microfluidics Mass Spectrometry Reveals Molecular Responses of Bacterial Filamentation and Inhibition Methods of Drug Resistant Bacteria
Description	Filamentation is one of the self-saving responses and a survival strategy of bacteria in the circumstance of antibiotics. Understanding the molecular mechanism of filamentation can lead to the development of new inhibition methods for antibiotic-resistant bacteria. In this work, we develop an online microfluidics mass spectrometry for real‐time analysis of metabolic changes of bacteria during filamentation under the stimulus of antibiotics. Together with untargeted proteomic analyses and fluorescence imaging of target enzymes, significant pathways, e.g. nucleotide metabolism and coenzyme A biosynthesis, correlated to the filamentation of extended spectrum beta‐lactamase producing E. coli (ESBL-E. coli) are identified.
HostingRepository	iProX
AnnounceDate	2021-02-26
AnnouncementXML	Submission_2024-03-20_00:11:30.420.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongxue Zhang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-25 23:33:07	ID requested
⏵ 1	2024-03-20 00:11:31	announced

Zhang D, Yin F, Qin Q, Qiao L, Molecular responses during bacterial filamentation reveal inhibition methods of drug-resistant bacteria. Proc Natl Acad Sci U S A, 120(27):e2301170120(2023) [pubmed]

submitter keyword: Bacterial filamentation, bacteria inhibition, multi-omics.

Liang Qiao
contact affiliation	Fudan University
contact email	liang_qiao@fudan.edu.cn
lab head
Dongxue Zhang
contact affiliation	Fudan University
contact email	16210220015@fudan.edu.cn
dataset submitter

iProX dataset URI