PXD024375

PXD024375 is an original dataset announced via ProteomeXchange.

Title	Allicin inhibits SARS-CoV-2 replication and abrogates the antiviral host response in the Calu-3 proteome
Description	The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic causes a major health burden. Garlic organosulfur compounds, such as the diallyl thiosulfinate allicin exert strong antimicrobial activity against various respiratory pathogens. Here, we investigated the antiviral activity of allicin against SARS-CoV-2 in infected Vero E6 and Calu-3 lung cells. Allicin efficiently inhibited viral replication and infectivity in both cell lines. Proteome analyses of infected Calu-3 cells revealed a strong induction of the antiviral interferon-stimulated gene (ISG) signature (e.g. Mx1, IFIT, 2’5’OAS and ISG15), pathways of vesicular transport, tight junctions (KIF5A/B/C, OSBPL2, CLTC1, ARHGAP17) and ubiquitin modification (UBE2L3/5), as well as reprogramming of host metabolism, transcription and translation. Allicin abrogated the ISG host response and reverted the host cellular pathways to Mock levels, confirming the antiviral and immunomodulatory activity of allicin in the host proteome. Thus, biocompatible doses of garlic could be promising for protection of lung cells against SARS-CoV-2.
HostingRepository	PRIDE
AnnounceDate	2021-06-30
AnnouncementXML	Submission_2021-06-29_21:25:13.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorenz Adrian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 22:22:29	ID requested
⏵ 1	2021-06-29 21:25:13	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2/ allicin/ Vero E6/ Calu-3/ proteome

Antelmann, Haike
contact affiliation	Institute for Biology-Microbiology Freie Universität Berlin Königin-Luise-Strasse 12-16 D-14195 Berlin Germany
contact email	haike.antelmann@fu-berlin.de
lab head
Lorenz Adrian
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	lorenz.adrian@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024375
     1. Label: PRIDE project
     2. Name: Allicin inhibits SARS-CoV-2 replication and abrogates the antiviral host response in the Calu-3 proteome