PXD024361 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of the ciliary Hedgehog response reveals coordinated exit of GPR161 and PKA |
Description | The primary cilium is a signaling compartment that interprets Hedgehog signals through changes of its protein, lipid and second messenger compositions. Here, we combine proximity labeling of cilia with quantitative mass spectrometry to unbiasedly profile the time-dependent alterations of the ciliary proteome in response to Hedgehog. This approach correctly identifies the three factors known to undergo Hedgehog-regulated ciliary redistribution and reveals two such additional proteins. First, we find that a regulatory subunit of the cAMP-dependent protein kinase (PKA) rapidly exits cilia together with the G protein-coupled receptor GPR161 in response to Hedgehog; and we propose that the GPR161/PKA module senses and amplifies cAMP signals to modulate ciliary PKA activity. Second, we identify the phosphatase Paladin as a cell type-specific regulator of Hedgehog signaling that enters primary cilia upon pathway activation. The broad applicability of quantitative ciliary proteome profiling promises a rapid characterization of ciliopathies and their underlying signaling malfunctions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-07 |
AnnouncementXML | Submission_2021-04-06_22:48:54.046.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marian Kalocsay |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-24 08:30:40 | ID requested | |
⏵ 1 | 2021-04-06 22:48:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Primary Cilium, protein trafficking, proximity labeling, proteomics, hedgehog signaling |
Contact List
David Mick |
contact affiliation | Center of Human and Molecular Biology (ZHMB), Saarland University School of Medicine, Homburg, Germany |
contact email | david.mick@uks.eu |
lab head | |
Marian Kalocsay |
contact affiliation | Harvard Medical School |
contact email | marian_kalocsay@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024361
- Label: PRIDE project
- Name: Proteomic profiling of the ciliary Hedgehog response reveals coordinated exit of GPR161 and PKA