PXD024350

PXD024350 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of Ataxin-2 interactome using LC-MS/MS
Description	We analyzed Ataxin-2 interactome using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). HEK293T cell lysate was subjected to immunoprecipitation (IP) with either anti-Ataxin-2 antibody or control IgG. The interaction between Ataxin-2 and the copurified proteins do not appear to be mediated by RNA, as the immunoprecipitation experiments were performed under buffer conditions that include RNase A and RNase I. The proteins purified using anti-Ataxin-2 and control IgG were separated by SDS-PAGE. CBB-stained gel was divided into three parts per lane in order to increase the detection sensitivity. Proteins extracted from the gels were analyzed by liquid chromatography coupled to tandem mass spectrometry to identify their protein content.
HostingRepository	PRIDE
AnnounceDate	2021-03-22
AnnouncementXML	Submission_2021-03-21_22:57:32.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroto Inagaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 02:53:04	ID requested
⏵ 1	2021-03-21 22:57:33	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Human, Ataxin-2, LC-MSMS

submitter keyword: Human, Ataxin-2, LC-MSMS

Contact List

Shin-ichi Hoshino
contact affiliation	Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University
contact email	hoshino@phar.nagoya-cu.ac.jp
lab head
Hiroto Inagaki
contact affiliation	Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University
contact email	hiroto170613@gmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024350

PRIDE project URI

Repository Record List

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