PXD024349

PXD024349 is an original dataset announced via ProteomeXchange.

Title	LC-MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity
Description	An LC-MS based discovery proteomics approach was used to measure the nuclear proteome fractions of Arabidopsis thaliana cell culture. An enrichment score based on the relative abundance of cytoplasmic, mitochondrial and golgi markers in the nuclear protein fraction allowed us to curate the nuclear proteome producing high quality catalogs of around 3,000 nuclear proteins under untreated and both PTI conditions (flg22 and nlp20). The measurements also covered low abundant proteins including more than 100 transcription factors and transcriptional co-activators. Here we sought to gain a broader impression of protein import to the nucleus upon stimulus with flg22 and nlp20. Furthermore, the abundance of 93 proteins changed significantly in the nucleus following elicitation of immunity. These results suggest promiscuous ribosome assembly and retrograde signaling from the mitochondrion to the nucleus including Prohibitins and Cytochrome C, in the two forms of PTI.
HostingRepository	PRIDE
AnnounceDate	2023-02-27
AnnouncementXML	Submission_2023-02-27_14:01:20.626.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SteffenNeumann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 02:37:47	ID requested
1	2021-11-08 09:28:34	announced
⏵ 2	2023-02-27 14:01:21	announced	2023-02-27: Updated project metadata.

Ayash M, Abukhalaf M, Thieme D, Proksch C, Heilmann M, Schattat MH, Hoehenwarter W, Nuclear Proteome and Protein Import in Pattern Triggered Immunity. Front Plant Sci, 12():744103(2021) [pubmed]

submitter keyword: Arabidopsis thaliana

Nucleus

Nuclear proteome

stress response

flg22

nlp20;

WolfgangHoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry Research Group Proteome Biology of Plant Interactions
contact email	Wolfgang.Hoehenwarter@ipb-halle.de
lab head
SteffenNeumann
contact affiliation	IPB Halle
contact email	sneumann@ipb-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024349

PRIDE project URI

• PRIDE
  1. PXD024349
     1. Label: PRIDE project
     2. Name: LC-MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity