PXD024345

PXD024345 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multiplexed quantitative proteomics in an autoimmune mice model of myocarditis (EAM)
Description	We performed a high-throughput, multiplexed quantitative proteomics analysis to determine protein abundance changes produced by BML-111 treatment in EAM and Ctrl mice. EAM was induced in 7 weeks-old BALB/c female mice by immunization at days 0 and 7 with a subcutaneous injection of 350 μg of murine cardiac myosin (MyHCα) in a 1:1 emulsion with complete Freund’s Adjuvant (SIGMA). MyHCα was isolated from hearts of Balb/c mice; control mice were injected with a 1:1 emulsion of physiological saline solution with complete Freund’s Adjuvant. Mice were treated daily for two weeks from day 7 by intraperitoneal injection with two complementary treatments: 1mg/Kg BML-111(Enzo Life Science) or its vehicle (2.5% ethanol). The proteomics analysis was performed in heart tissue protein extracts (four animals per group: Ctrl+Veh, EAM+Veh, Ctrl+BML and EAM+BML.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:51.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inmaculada Jorge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 02:18:14	ID requested
1	2022-12-07 02:19:20	announced
⏵ 2	2023-11-14 08:26:53	announced	2023-11-14: Updated project metadata.

Publication List

Val-Blasco A, Prieto P, Ja, é, n RI, Gil-Fern, á, ndez M, Pajares M, Domenech N, Terr, ó, n V, Tamayo M, Jorge I, V, á, zquez J, Bueno-Sen A, Vallejo-Cremades MT, Pombo-Otero J, Sanchez-Garc, í, a S, Ruiz-Hurtado G, G, ó, mez AM, Zaragoza C, Crespo-Leiro MG, L, ó, pez-Collazo E, Cuadrado A, Delgado C, Bosc, á L, Fern, á, ndez-Velasco M, Handling and NRF2 Activation. JACC Basic Transl Sci, 7(6):544-560(2022) [pubmed]

Val-Blasco A, Prieto P, Ja, é, n RI, Gil-Fern, á, ndez M, Pajares M, Domenech N, Terr, ó, n V, Tamayo M, Jorge I, V, á, zquez J, Bueno-Sen A, Vallejo-Cremades MT, Pombo-Otero J, Sanchez-Garc, í, a S, Ruiz-Hurtado G, G, ó, mez AM, Zaragoza C, Crespo-Leiro MG, L, ó, pez-Collazo E, Cuadrado A, Delgado C, Bosc, á L, Fern, á, ndez-Velasco M, Handling and NRF2 Activation. JACC Basic Transl Sci, 7(6):544-560(2022) [pubmed]

Keyword List

submitter keyword: heart,Mouse, LC-MS/MS

submitter keyword: heart,Mouse, LC-MS/MS

Contact List

Jesús María Vázquez Cobos
contact affiliation	Centro Nacional de Investigaciones Cardiovasculares (CNIC) and CIBER de Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain
contact email	jvazquez@cnic.es
lab head
Inmaculada Jorge
contact affiliation	Vascular Biology and Inflammation
contact email	ijorge@cnic.es
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD024345

PRIDE project URI

Repository Record List

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