PXD024336

PXD024336 is an original dataset announced via ProteomeXchange.

Title	Newly translated protein misfolding after heat shock and translation during heat shock
Description	Correct and efficient folding of nascent proteins to their native state requires support from the protein homeostasis network. We set to examine which newly translated proteins are less thermostable to infer which polypeptides require more time to fold. More specifically, we sought to determine which newly translated proteins are more susceptible to misfolding and aggregation under heat stress using pulse SILAC. These proteins were abundant, shorter, and highly ordered, with a potentially larger hydrophobic core as suggested by their higher hydrophobicity. Notably these proteins contain more β-sheets that typically require more time for folding and were enriched for Hsp70/Ssb and TRiC/CCT binding motifs, suggesting a higher demand for chaperone-assisted folding. These polypeptides were also more often components of stable protein complexes. All evidence combined suggests that a specific subset of newly translated proteins requires more time following synthesis to reach a thermostable native state in the cell.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:11.770.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mang Zhu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-24 02:11:07	ID requested
1	2022-08-11 16:26:25	announced
⏵ 2	2023-11-14 08:52:12	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics,Protein folding, protein aggregation, proteostasis, heat stress

Thibault Mayor
contact affiliation	Michael Smith Laboratories, The University of British Columbia
contact email	mayor@msl.ubc.ca
lab head
Mang Zhu
contact affiliation	UBC
contact email	mang.zhu@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024336
     1. Label: PRIDE project
     2. Name: Newly translated protein misfolding after heat shock and translation during heat shock