PXD024335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Autophagosome content profiling - STAR Protocol Data Set |
Description | The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autophagosomes and potentially other transient vesicles without the need of subcellular fractionation. By combining live-cell biotinylation with proteinase K digestion of cell homogenates, proteins enriched in membrane-protected compartments can be readily enriched and identified. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-06 |
AnnouncementXML | Submission_2021-04-06_03:10:00.023.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Behrends |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-24 01:16:50 | ID requested | |
⏵ 1 | 2021-04-06 03:10:00 | announced | |
Publication List
Zellner S, Schifferer M, Behrends C, Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. Mol Cell, 81(6):1337-1354.e8(2021) [pubmed] |
Keyword List
submitter keyword: Human, APEX2, proximity proteomics, proteinase K digest |
Contact List
Christian Behrends |
contact affiliation | Munich Cluster for Systems Neurology (SyNergy), Ludwig-Maximilians-Universität München |
contact email | christian.behrends@mail03.med.uni-muenchen.de |
lab head | |
Christian Behrends |
contact affiliation | SyNergy, LMU München |
contact email | christian.behrends@mail03.med.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024335 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024335
- Label: PRIDE project
- Name: Autophagosome content profiling - STAR Protocol Data Set