PXD024334
PXD024334 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome- and N-terminome-driven annotation of proteolysis in beige adipocyte maturation. |
Description | Beige adipocytes have the reversible thermogenic capacity with increasing Ucp1 expression in response to environmental stimuli. Beige adipocytes acquire thermogenic function during maturation. And their maturation, including changes in morphology and functions, remodeling of the extracellular matrix and organelles turnover, are associated with the proteolytic process. To inspect the post-translational events related to beige adipocyte maturation, we analyze the global proteome and N-terminome to profile proteases and proteolysis products. We induced the differentiation of the preadipocyte cells isolated from the inguinal white adipose tissue into beige adipocytes and harvested the cells every two days. For bottom-up proteome and N-terminome profiling, cell lysates from five time points were subjected to TrypN digestion. N-terminal peptides of the proteolysis products were isolated using the N-terminomics approach based on our TrypN-SCX strategy. In this case, StageTip-based SCX chromatography was used for this large-scale analysis |
HostingRepository | jPOST |
AnnounceDate | 2021-05-10 |
AnnouncementXML | Submission_2022-09-18_03:51:01.702.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chih-Hsiang Chang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-02-24 00:36:25 | ID requested | |
1 | 2021-05-09 22:46:35 | announced | |
2 | 2021-05-09 23:59:08 | announced | 2021-05-10: Updated PubMed. |
⏵ 3 | 2022-09-18 03:51:02 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: adipocytes maturation, N-terminomice, SCX |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Chih-Hsiang Chang | |
contact affiliation | Kumamoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001099/ |