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PXD024334

PXD024334 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome- and N-terminome-driven annotation of proteolysis in beige adipocyte maturation.
DescriptionBeige adipocytes have the reversible thermogenic capacity with increasing Ucp1 expression in response to environmental stimuli. Beige adipocytes acquire thermogenic function during maturation. And their maturation, including changes in morphology and functions, remodeling of the extracellular matrix and organelles turnover, are associated with the proteolytic process. To inspect the post-translational events related to beige adipocyte maturation, we analyze the global proteome and N-terminome to profile proteases and proteolysis products. We induced the differentiation of the preadipocyte cells isolated from the inguinal white adipose tissue into beige adipocytes and harvested the cells every two days. For bottom-up proteome and N-terminome profiling, cell lysates from five time points were subjected to TrypN digestion. N-terminal peptides of the proteolysis products were isolated using the N-terminomics approach based on our TrypN-SCX strategy. In this case, StageTip-based SCX chromatography was used for this large-scale analysis
HostingRepositoryjPOST
AnnounceDate2021-05-10
AnnouncementXMLSubmission_2022-09-18_03:51:01.702.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChih-Hsiang Chang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-24 00:36:25ID requested
12021-05-09 22:46:35announced
22021-05-09 23:59:08announced2021-05-10: Updated PubMed.
32022-09-18 03:51:02announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: adipocytes maturation, N-terminomice, SCX
Contact List
Yasushi Ishihama
lab head
Chih-Hsiang Chang
contact affiliationKumamoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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