PXD024332

PXD024332 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide effects of single gene perturbations in a eukaryotic cell
Description	Protein expression levels are controlled at the transcriptional, translational and post-translational level and their regulatory principles are starting to emerge. While transcriptional outcomes, which are commonly also used as a proxy for protein abundance, have been investigated on a larger scale, the study of translational output requires large-scale proteomics data. However, data for proteome alterations by systematic assessment of knockouts genome-wide is not available yet. We here determined the individual proteome changes for 3,308 non-essential genes in the yeast S. pombe. We observed that genes with high proteome remodeling are predominantly involved in gene expression regulation, in particular acting as translational regulators. Focusing on those knockout strains with a large number of altered proteins, we performed paired transcriptome/proteome measurements to uncover translational regulators and features of translational regulation. Furthermore, by similarity clustering of these proteome changes, we infer gene functionality that can be extended to other species such as human or baker’s yeast.
HostingRepository	PRIDE
AnnounceDate	2022-09-22
AnnouncementXML	Submission_2022-09-22_01:42:10.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Schizosaccharomyces pombe OY26; NCBI TaxID: 649908;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 23:33:48	ID requested
⏵ 1	2022-09-22 01:42:10	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS

knockout strain

Falk Butter
contact affiliation	IMB, Ackermannweg 4, 55128 Mainz
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD024332

PRIDE project URI

• PRIDE
  1. PXD024332
     1. Label: PRIDE project
     2. Name: Proteome-wide effects of single gene perturbations in a eukaryotic cell