PXD024329

PXD024329 is an original dataset announced via ProteomeXchange.

Title	Quantification of poly-ubiquitinated proteins in wild type Arabidopsis and amiNAA10 plants
Description	Wild type Arabidopsis Col-0 plants and mutants depleted of the catalytic subunit of the ribosome-associated N-acetyltransferase A (NatA) complex (amiNAA10) were grown on soil for six weeks under short-day conditions (8h light, light intensity: 100 µE, 21 °C/18 °C temperature (day/night), 50 % humidity). Proteasome and deubiquitinase activity were inhibited at 10 am (2 h of light) by floating leaf discs on ½ Hoagland supplemented with 50 µM MG132 and 20 µM PR-619 for 5 h.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:23:58.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 23:09:49	ID requested
⏵ 1	2022-05-25 09:23:59	announced

Linster E, Forero Ruiz FL, Miklankova P, Ruppert T, Mueller J, Armbruster L, Gong X, Serino G, Mann M, Hell R, Wirtz M, Cotranslational N-degron masking by acetylation promotes proteome stability in plants. Nat Commun, 13(1):810(2022) [pubmed]

submitter keyword: Arabidopsis thaliana, N-acetyltransferase A, N-terminal protein acetylation, co-translational protein modification, post-translational protein modification, Ubiquitin-proteasome system, protein degradation, N-degron pathways, nonAc-X²/N-degron

Thomas Ruppert
contact affiliation	Core facility for mass spectrometry and proteomics, ZMBH, University Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD024329

PRIDE project URI

• PRIDE
  1. PXD024329
     1. Label: PRIDE project
     2. Name: Quantification of poly-ubiquitinated proteins in wild type Arabidopsis and amiNAA10 plants