PXD024326

PXD024326 is an original dataset announced via ProteomeXchange.

Title	Interactome of Toxoplasma gondii Pre-mRNA 3’ Processing Complexes
Description	A proper 3’end processing of mRNAs is regarded as one of the regulatory cornerstones of gene expression. In a parasite that must answer to the high regulatory requirements of its multi-host life style, the need is grand for adopting additional means to partition the distinct transcriptional signatures of the closely and tandemly-arranged stage specific genes. In this study, we report on our findings in T. gondii of an m6A-dependent 3’end polyadenylation serving as a transcriptional barrier at these loci. We identify the core polyadenylation complex within T. gondii and establish CPSF4 as a reader for m6A-modified mRNAs, via a YTH domain within its C-terminus, a feature which is shared with plants.
HostingRepository	PRIDE
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2021-07-15_23:53:26.980.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024326
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 23:00:09	ID requested
⏵ 1	2021-07-15 23:53:27	announced

Farhat DC, Bowler MW, Communie G, Pontier D, Belmudes L, Mas C, Corrao C, Cout, é Y, Bougdour A, Lagrange T, Hakimi MA, Swale C, . Elife, 10():(2021) [pubmed]

submitter keyword: Toxoplasma gondii, pre-mRNA processing, 3'UTR, interactomics, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024326

PRIDE project URI

• PRIDE
  1. PXD024326
     1. Label: PRIDE project
     2. Name: Interactome of Toxoplasma gondii Pre-mRNA 3’ Processing Complexes