PXD024319

PXD024319 is an original dataset announced via ProteomeXchange.

Title	Rv0180c contributes to M. tuberculosis cell shape and to infectivity in mice and host cells
Description	Mycobacterium tuberculosis, the main causative agent of human tuberculosis, is transmitted from person to person via small droplets containing very few bacteria. Optimizing the chance to seed in the lung is therefore a major adaptation to favor survival and dissemination in the human population. Here we used TnSeq to identify genes important for the early events leading to bacterial seeding in the lung. We found several genes encoding known virulence factors and 3 new candidates not previously described: rv0180c, rv1179c and rv1592c. We focused on the gene, rv0180c, of unknown function and demonstrated its requirement for seeding in the lung of mice and for initiating the infection. We found that Rv0180c enhances entry into macrophages and epithelial cells. In human macrophages, this effect is mediated by the complement-receptor 3, CR3, a major phagocytic receptor for M. tuberculosis. Blocking the CR3 lectin site abolished the difference between the wild-type parental strain and the rv0180c::km mutant with regard to bacterial entry into macrophages. However, we did not detect any difference in the production of both CR3-known ligands (glucan, arabinomannan, mannan), CR3-modulating lipids (phthiocerol dimycocerosate) or proteins in the outermost layer of the rv0180c::km mutant in comparison to the wild-type or complemented strains. We established that Rv0180c contributes to the functionality of the bacterial cell envelope regarding resistance to toxic molecule attack and cell shape. This alteration of bacterial shape may explain the lower engagement of receptors and open a new perspective on the modulation of bacterial infectivity.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_15:57:44.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre STELLA
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv_2009; NCBI TaxID: 747078;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-23 04:21:32	ID requested
⏵ 1	2022-02-16 15:57:45	announced

Payros D, Alonso H, Malaga W, Volle A, Maz, è, res S, D, é, jean S, Vali, è, re S, Moreau F, Balor S, Stella A, Combes-Soia L, Burlet-Schiltz O, Bouchez O, Nigou J, Astarie-Dequeker C, Guilhot C, Rv0180c contributes to Mycobacterium tuberculosis cell shape and to infectivity in mice and macrophages. PLoS Pathog, 17(11):e1010020(2021) [pubmed]

submitter keyword: Mycobacterium tuberculosis H37Rv , LC-MS/MS

Christophe Guilhot
contact affiliation	IPBS, CNRS-UPS, BP 64182, 205 route de Narbonne, F-31077 Toulouse Cedex 04, France
contact email	Christophe.Guilhot@ipbs.fr
lab head
Alexandre STELLA
contact affiliation	CNRS
contact email	alexandre.stella@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024319
     1. Label: PRIDE project
     2. Name: Rv0180c contributes to M. tuberculosis cell shape and to infectivity in mice and host cells