PXD024293

PXD024293 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of microplasts derived from macrophage conditioned medium treated breast adenocarcinoma MCF-7 cells. Pooja Kamal Melwani, M.Sc., Nishad S, PhD, Badri Narain Pandey, PhD, Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
Description	Paracrine signals relayed between heterogenous cell types through small and large extracellular vesicles promote cancer cell growth, invasion and metastasis. Microplasts, also called as cytoplasts or cytokineplasts are large extracellular vesicles (0.5-11µm diameter) originating from migratory cells. Microplasts are known to be associated with invasive phenotypes of cancer cells and promote metastasis. Treatment with macrophage conditioned medium induces significant shedding of microplasts from MCF-7 breast adenocarcinoma cells. To characterise microplasts and to study their potential role in intercellular communication in cancer, we isolated microplasts derived from macrophage conditioned medium treated MCF-7 cells and investigated their protein cargo through LC-MSMS.
HostingRepository	PRIDE
AnnounceDate	2021-05-05
AnnouncementXML	Submission_2021-05-19_07:31:54.658.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024293
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pooja Melwani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-22 01:45:03	ID requested
1	2021-05-05 06:04:20	announced
⏵ 2	2021-05-19 07:31:55	announced	2021-05-19: Updated publication reference for PubMed record(s): 33993482.

Melwani PK, Balla MMS, S N, Padwal M, Chaurasia RK, Basu B, Ghosh A, Pandey BN, Integrated transcriptomic and proteomic analysis of microplasts derived from macrophage-conditioned medium-treated MCF-7 breast cancer cells. FEBS Lett, 595(13):1844-1860(2021) [pubmed]

submitter keyword: Microplasts, Cytoplasts, Large extracellular vesicles, breast cancer, MCF-7, LC-MSMS

Badri Narain Pandey, PhD
contact affiliation	Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
contact email	bnp@barc.gov.in
lab head
Pooja Melwani
contact affiliation	Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India
contact email	pooja.patheja8@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024293

PRIDE project URI

• PRIDE
  1. PXD024293
     1. Label: PRIDE project
     2. Name: LC-MSMS of microplasts derived from macrophage conditioned medium treated breast adenocarcinoma MCF-7 cells. Pooja Kamal Melwani, M.Sc., Nishad S, PhD, Badri Narain Pandey, PhD, Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India