PXD024284

PXD024284 is an original dataset announced via ProteomeXchange.

Title	Reduced thrombogenicity of surface-treated Nitinol implants steered by altered protein adsorption
Description	Blood-contacting medical implants made of Nitinol and other titanium alloys, such as neurovascular flow diverters and peripheral stents, have the disadvantage of being highly thrombogenic. This makes the use of systemic (dual) anti platelet / anticoagulant therapies inevitable with related risks of device thrombosis, bleeding and other complications. Meeting the urgent clinical demand for a less thrombogenic Nitinol surface, we describe here a treatment of standard, commercially available Nitinol that renders its surface ultra-hydrophilic and functionalized with phosphate ions. The efficacy of this treatment was assessed by comparing standard and surface-treated Nitinol disks and braids, equivalent to flow diverters. Static and dynamic (Chandler loop) blood incubation tests showed a drastic reduction of thrombus formation on treated devices. Surface chemistry and proteomic analysis indicated a key role of phosphate and calcium ions in steering blood protein adsorption and avoiding coagulation cascade activation and platelet adhesion. A good endothelialization of the surface confirmed the biocompatibility of the treated surface.
HostingRepository	PRIDE
AnnounceDate	2022-01-21
AnnouncementXML	Submission_2022-01-24_05:55:27.951.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katharina Gegenschatz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-22 01:44:15	ID requested
1	2022-01-21 06:56:08	announced
⏵ 2	2022-01-24 05:55:28	announced	2022-01-24: Updated publication reference for PubMed record(s): 34673227.

Gegenschatz-Schmid K, Buzzi S, Grossmann J, Roschitzki B, Urbanet R, Heuberger R, Gl, ü, ck D, Zucker A, Ehrbar M, Reduced thrombogenicity of surface-treated Nitinol implants steered by altered protein adsorption. Acta Biomater, 137():331-345(2022) [pubmed]

submitter keyword: Antithrombogenic treatment, hemocompatibility, cardiovascular devices, intrinsic coagulation pathway, platelet adhesion, proteomics

Martin Ehrbar
contact affiliation	Head of Research Department of Obstetrics University Hospital Zurich Schmelzbergstrasse 12 PATH G48 / PF 125 CH-8091 Zürich Switzerland
contact email	martin.ehrbar@usz.ch
lab head
Katharina Gegenschatz
contact affiliation	University Hospital Zurich
contact email	katharina.gegenschatz@usz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD024284

PRIDE project URI

• PRIDE
  1. PXD024284
     1. Label: PRIDE project
     2. Name: Reduced thrombogenicity of surface-treated Nitinol implants steered by altered protein adsorption